Add different volumes of buffer, and different buffer from a divided reservoir, to a 96wp

Agilent
1

Hi everyone! We need to dilute (normalize) a 96wp with samples at different concentrations, to a final concentration but the buffers needed to do that are different per sample (there will be a max of 4 buffer types distributed amongs samples). I am trying to do hit pick replication, but it won’t let me do it from a reservoir (divided reservoir in my case), to a 96wp plate, unless I am missing something?

One workaround would simply be to trick the robot and to just use a 96wp on VWORKS and properly adjust the asp distance etc, but on the deck a reservoir will be present.

But I’d like the software to actually have the correct labware, instead of tricking it.

Is there a way to do that?

thanks!

2

The hit pick will not do what you need. If I understand your request correctly, you’ll be having on the deck the following plates:

  • clean tips
  • used tips (generally starting empty)
  • samples to be diluted
  • destination plate
  • buffer plate (you mentioned a multi-vessel reservoir if I understand).

Under the hypothesis that you want to use the head A1 for single-tip liquid handling I would suggest the following:

loc 4 - tips on position

loc 1 - tips off position

loc 2 - sample plate with samples

loc 5 - destination plate with the normalized samples

loc 7 or 8 or 9 - multi column reservoir with multiple buffers. Buffers will be aspirated using any position in the A row.

A suitable input file will be prepared with the following columns:

col1 - starting well in the sample plate

col2 - volume of sample to be transferred

col3 - destination well in the destination plate (if needed)

col4 - buffer well address (for example for a 12-col reservoir A1 for col 1, A2 for col2 etc…)

col 5 - volume of buffer.

The protocol will parse the input file and create a data structure (for example an array of objects, where each object represents a line of the input file).

The protocol will have two subprocesses (or equivalently, but better, two processes that can be independently called), one subprocess that transfers the buffer to the wells and another subprocess for transferring the samples and mixing.

Tips will be managed using the “tip state editor”.

Could this work for you?

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