Is there a list of error codes that are recorded somewhere for easy reference?
We are trying to use the ALC to automatically train liquid heights for volumes <10uL but run into Main Error Code: 62 when adjusting i_int_ZLimit in the SearchForCLLD_Z function. Default value is set to 3. Setting to 1 gives Main Error Code: 70 which is cLLD related, however setting the value to 0 gives us a new code that we have not seen before.
Pictures attached below for reference. Thank you!
Hi @yhs.allen ,
Error 62 is a Z step loss error. Setting the i_int_ZLimit parameter only affects the amount of pushback the channels allow. Setting it to 0 on Vantage basically set this to the most sensitive level so the base tension of the channel likely threw the step loss. I would recommend not tweaking anything in the ALC method otherwise you risk damaging your channels.
Unless the labware is a PCR plate and is sitting on the 96 well PCR module, you are likely not going to be able to define heights for volumes < 10uL. For that method, 10uL is likely the lower limit depending on the labware in question.
The error 70 (No liquid level found) is already an indication that the volume is below the detection level for the teaching needle.
Thank you that makes sense.
This actually is an Eppendorf TwinTec PCR plate and so we are trying to train on volumes less than 10uL. Would this need to be done manually or can we still use the ALC on this since it’s a PCR?
We initially just removed the minimum height allowed via the custom dialog from 10uL down to 1uL but ran into the same SearchForCLLD_Z error so we assumed it had to do with limitations of the function itself.
Hi @yhs.allen ,
I would recommend manually setting those values, although the data may not be as useful. The difference in height from 10uL to say 9uL is likely smaller than the minimum movements that the channels can be return (0.1 mm step distance). You are also going to start running into detection limitations sub 10uL as well. Even with very conductive liquids, the grounding starts to break down.
What would be the root cause if we go from 10uL to 5uL and still run into an Error 62? Because this gap is >0.1mm I don’t see it being because of that.
What’s this dialog? Part of the new Venus or just a smt I haven’t encountered before?
Hi @yhs.allen ,
5uL is not very large so the channel is bottoming out before it detects the liquid.
@BrandonBare_Hamilton I would also like to know where to find a list that describes all error codes. Please share, if possible.
Hi @HenningGramHansen ,
If you right click on any of the ML_STAR step commands and select What’s this? it will open the help menu for that command.
From there you can click on the General step result format… link.
In that page click on the MainErr link to open up a list of all the Main errors.
These are the error codes that you are going to encounter with normal operation of your system and should match the descriptions that the error handling dialog shows.
Thanks - that is very informative! Are you able to provide a similar list with a description of the “SlaveErr” codes?
Here is a list of STARline error codes: STARline Errors.xlsx :: Hamilton
Thank you,
Dan
