Would you mind sharing the tip type that you are using (size, bore) and arm (FCA, MCA)?
Also if you can share the LC xml in a message, I’m happy to review it when I have some downtime during lunch hour. In the past I’ve been mindful of items like backlash compensation or even the speed of retraction from the container.
One thing I will add is that at smaller volumes, I’m hard pressed to trust data coming directly from companies. For starters, my lab is different than their testing labs. I’ve seen things like elevation, proximity to large bodies of water, or even floor level impact systems. Don’t get me started on HVAC systems. Second, they are experts at their platforms and can reach out to the folks who built the components (if they did so in-house) or can exploit novel features.
At something like 2uL, I would aim for a much broader CV % or at least push for guardbanding studies of ranges if possible. It is true that our LC testing is generally more specific to the moment (place & time) of the conditions, things can or will evolve and may be different at 11am vs 2am in 24/7 labs. Knowing the impact of a range on results is useful for troubleshooting but also can help inform your true CV % for operational efficiency.
With that said, I found some useful threads on glycerol strategies.
https://labautomation.io/t/50-glycerol-w-fca-mca/1806/2
https://labautomation.io/t/glycerol-lc-help/3132
https://labautomation.io/t/advice-needed-efficient-glycerol-hit-picking-with-hamilton-microlab-prep/3535/5
Not sure if @Kastronaut or @dirkvanessen can share more here