Tecan Fluent FCA Plasma Diluted in 50% Glycerol Small Volume Contact Dispense Help

Thanks @luisvillaautomata for the shout - I’ll see what I can recall immediately and check my notes next week.

We have a liqFCA with the metal 1mL tips, and in testing my solution (50% glycerol, 50% aqueous) we had to keep it chilled while performing the run. The really slow speeds and introducing a wash step every so often (once in the beginning, once after any mix, and another at the end for good measure) got us pretty close. It resets the system trailing air gap and that proved to help me a great deal.

What @HarenArul mentions in this comment on my post from back in 2023 about overdispensing intentionally, only to aspirate off any residual droplets formed is interesting and would require some additional testing, but it definitely seems like a solid play overall.

IIRC my lowest volume was also 2 uL, and I don’t believe we needed to hit below 5% CV or inaccuracy.

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