2 Channels Performing Wrong Liquid Class

Hamilton (Venus)
1

The front two channels on my eight-channel are not doing the same liquid class as the rest of the channels. What could be causing this?

2

I’m not aware of any expected behavior that would explain this issue outside of a potential programming error. Please provide a method, trace file, and usb com trace file so we can help troubleshoot!

1 Like
3

Here’s a video, notice the front two channels are not mixing. This behavior occurs every time. The channels perform normal aspirations as expected.

Trace file:

2025-05-07 14:41:53.339 Microlab STARlet : 1000ul Channel Aspirate (Single Step) - start;
2025-05-07 14:42:06.027 Microlab STARlet : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: culture_plate, A1, 10 uL > channel 2: culture_plate, B1, 10 uL > channel 3: culture_plate, C1, 10 uL > channel 4: culture_plate, D1, 10 uL > channel 5: culture_plate, E1, 10 uL > channel 6: culture_plate, F1, 10 uL > channel 7: culture_plate, G1, 10 uL > channel 8: culture_plate, H1, 10 uL
2025-05-07 14:42:06.027 Microlab STARlet : 1000ul Channel Get Last Liquid Level (Single Step) - start;
2025-05-07 14:42:06.074 Microlab STARlet : 1000ul Channel Get Last Liquid Level (Single Step) - complete;  > channel 1:  0.0 mm > channel 2:  0.0 mm > channel 3:  0.0 mm > channel 4:  0.0 mm > channel 5:  0.0 mm > channel 6:  0.0 mm > channel 7:  0.0 mm > channel 8:  0.0 mm
2025-05-07 14:42:06.137 USER : Trace - complete; Aspirate step return 1:0[01,00,00,0,10.0,culture_plate,A1[02,00,00,0,10.0,culture_plate,B1[03,00,00,0,10.0,culture_plate,C1[04,00,00,0,10.0,culture_plate,D1[05,00,00,0,10.0,culture_plate,E1[06,00,00,0,10.0,culture_plate,F1[07,00,00,0,10.0,culture_plate,G1[08,00,00,0,10.0,culture_plate,H1
2025-05-07 14:42:06.137 USER : Trace - complete; Aspirate step return 2:0.000000;0.000000;0.000000;0.000000;0.000000;0.000000;0.000000;0.000000
2025-05-07 14:42:06.137 USER : Trace - complete; Aspirate step return 3:-1.658288;-1.658288;-1.658288;-1.658288;-1.658288;-1.658288;-1.658288;-1.658288

Firmware commands:

< 14:41:53.496 8AF#8000#00: C0RXid0246
> 14:41:53.517 8AF#8000#00: C0RXid0246er00/00rx+03430
< 14:41:53.520 8AF#8000#00: C0ASid0247at0&tm1&xp03430&yp1460 1370 1280 1190 1100 1010 0920 0830th2450te2450lp2001&ch000&zl1881&zx1871&ip0000&it0&fp0000&av00100&as1000&ta050&ba0000&oa020&lm0&ll1&lv1&ld00&de0020&wt10&mv01000&mc01&mp000&ms0750&gi000&gj0gk0zu0113&zr09285&mh0000&zo000&po0100&lk0&ik0000&sd0500&se0500&sz0300&io0000&il00000&in0000&
> 14:42:05.871 8AF#8000#00: C0ASid0247er00/00
< 14:42:06.027 8AF#8000#00: C0RLid0248
> 14:42:06.074 8AF#8000#00: C0RLid0248er00/00lh+0000 +0000 +0000 +0000 +0000 +0000 +0000 +0000
< 14:42:27.908 8AF#8000#00: C0RXid0249
> 14:42:27.923 8AF#8000#00: C0RXid0249er00/00rx+03430
< 14:42:27.923 8AF#8000#00: C0DSid0250dm3&tm1&xp03430&yp1460 1370 1280 1190 1100 1010 0920 0830zx1871&lp2001&zl1881&ip0000&it0&fp0000&th2450te2450dv00100&ds0750&ss0560&rv000&ta050&ba0000&lm0&zo000&ll1&lv1&de0040&mv00000&mc00&mp000&ms0750&wt00&gi000&gj0gk0zu0113&dj00zr09285&mh0000&po0100&
> 14:42:33.526 8AF#8000#00: C0DSid0250er00/00
< 14:42:33.667 8AF#8000#00: C0RLid0251
> 14:42:33.714 8AF#8000#00: C0RLid0251er00/00lh+0000 +0000 +0000 +0000 +0000 +0000 +0000 +0000
4

Hi Stefan,

The mixing function (cycles, volume) is not part of the liquid class. Just the mix flow rate. The firmware calls appear to be correct in terms of the parameters. You can look at the plungers up above to see if they are at least moving and are consistent with the other channels. If they are, then it may be an issue with the stop discs on those channels.

I’ll follow up with you separately to gather more info and materials.

Thanks,

Eric

5

Hi @Stefan ,

Has the system passed daily maintenance?

6

I will give that a shot next time im in lab. Thanks!

1 Like