Ability to specify barcode and get back default sequence for the labware

Hamilton (Venus)
1

Hi - Hoping to get some help for a project on Vantage.

One thing I would like to do is to specify the barcode and get back the default sequence for the labware. I am doing 96-channel plate stamping.

I have the function that will give me the sequence by specifying the labware.
Labware ID → Default sequence

Where would I find a function that will give me the labware ID by specifying the assigned barcode?
Barcode → Labware ID

And is there a way of going directly from the assigned barcode to getting the default sequence or do I need to go:
Barcode → Labware ID → Default sequence

@MikeMueller @EricSindelar_Hamilton

1 Like
2

Hi @KellyTracy ,

You can use the VectorDatabaseHelper to perform all of these tasks.

image
image688×302 13.1 KB

image
image706×313 12.5 KB

As long as the barcode has been scanned or set, and sample tracking is maintained, you can return the labware ID or sequence by using a barcode. This works even if the plate was transported around the deck as sample tracking will know that the plate has moved.

There are also functions for finding barcodes of containers and the corresponding sequence of the specified barcodes.

image
image379×282 6.54 KB

4 Likes
3

@DDeschenes this is similar to what we were talking about!

2 Likes
4

Brandon,

Thanks for your help with this and providing the library. One follow-up question on getting this operational. I have the plate barcode scanned by the autoloader, and we have this function now taking in the barcode and outputting the sequence. We are setting the position of that sequence to 1. We are then using that same sequence in the next aspirate step as part of a 96-well plate aspirate process.

But we are getting an error that it is out of positions in the sequence. It needs 96 positions in the sequence for the aspirate (we are using the 96-head). Is this function just generating a single-well sequence for the plate or is it generating the default sequence for the 96-well plate (it’s a 96-well deep well plate).

It seems that the sequence coming out of this function isn’t 96 positions like I would expect. So wondering what is the output sequence of this function and how should I go about getting the default 96-well sequence of the plate based on the plate barcode alone?

Thank you,
Mike Mueller

1 Like
5

Hi @mueller68 ,

It should return all 96 positions of the labware. The example method confirms this.

Could you trace the sequence after you use the function to see if maybe the sorting is not correct for the MPH. Are the labware that you are using loaded on the deck layout prior to run control, or are the labware added at run time? If the latter, this could be the root cause as there is no default sequences for that labware.

Another option would be to use this function, where you feed in the array of wells for it to build the sequence.

image
image1153×313 16.2 KB

6

Hi Brandon,

I am pasting the relevant portion of the TraceView below. So it does output a 96-position sequence in the correct column format. I am using the sequence that is output from that barcode->command in the aspirate. And that is where I am getting that there are 0 available positions, even though I am setting it to position 1 so I wouldn’t expect that to happen. I am using a variable name in the sequence field of the aspirate command - I suspect the issue lies somewhere there. The variable name I am using in the sequence field of the aspirate command is “R1_sequence_from_labware_barcode” and it of type sequence and that is the output from the barcode-> sequence command.

It is correctly grabbing the 96-position sequence from the barcode → sequence conversion. I believe that sequence should be stored in the output o_seqPositions which in my case is “R1_sequence_from_labware_barcode”

2025-04-04 13:06:44.906 USER : Trace - complete; name = __temp
2025-04-04 13:06:44.906 USER : Trace - complete; current = 1
2025-04-04 13:06:44.906 USER : Trace - complete; count = 96
2025-04-04 13:06:44.906 USER : Trace - complete; total = 96
2025-04-04 13:06:44.906 USER : Trace - complete; max = 0
2025-04-04 13:06:44.906 USER : Trace - complete; used = 0
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = A1
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = B1
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = C1
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = D1
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = E1
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = F1
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = G1
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = H1
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = A2
2025-04-04 13:06:44.906 USER : Trace - complete; labwareId = R1_001, positionId = B2
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = C2
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = D2
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = E2
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = F2
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = G2
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = H2
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = A3
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = B3
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = C3
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = D3
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = E3
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = F3
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = G3
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = H3
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = A4
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = B4
2025-04-04 13:06:44.908 USER : Trace - complete; labwareId = R1_001, positionId = C4
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = D4
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = E4
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = F4
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = G4
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = H4
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = A5
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = B5
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = C5
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = D5
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = E5
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = F5
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = G5
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = H5
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = A6
2025-04-04 13:06:44.910 USER : Trace - complete; labwareId = R1_001, positionId = B6
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = C6
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = D6
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = E6
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = F6
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = G6
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = H6
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = A7
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = B7
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = C7
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = D7
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = E7
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = F7
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = G7
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = H7
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = A8
2025-04-04 13:06:44.912 USER : Trace - complete; labwareId = R1_001, positionId = B8
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = C8
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = D8
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = E8
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = F8
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = G8
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = H8
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = A9
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = B9
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = C9
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = D9
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = E9
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = F9
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = G9
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = H9
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = A10
2025-04-04 13:06:44.914 USER : Trace - complete; labwareId = R1_001, positionId = B10
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = C10
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = D10
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = E10
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = F10
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = G10
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = H10
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = A11
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = B11
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = C11
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = D11
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = E11
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = F11
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = G11
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = H11
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = A12
2025-04-04 13:06:44.916 USER : Trace - complete; labwareId = R1_001, positionId = B12
2025-04-04 13:06:44.948 USER : Trace - complete; labwareId = R1_001, positionId = C12
2025-04-04 13:06:44.948 USER : Trace - complete; labwareId = R1_001, positionId = D12
2025-04-04 13:06:44.948 USER : Trace - complete; labwareId = R1_001, positionId = E12
2025-04-04 13:06:44.980 USER : Trace - complete; labwareId = R1_001, positionId = F12
2025-04-04 13:06:44.980 USER : Trace - complete; labwareId = R1_001, positionId = G12
2025-04-04 13:06:44.980 USER : Trace - complete; labwareId = R1_001, positionId = H12
2025-04-04 13:06:45.033 Microlab® STAR / VANTAGE : CO-RE 96 Head Aspirate - start;
2025-04-04 13:06:45.047 Microlab® STAR / VANTAGE : CO-RE 96 Head Tip Pick Up (Single Step) - start;
2025-04-04 13:06:54.138 Microlab® STAR / VANTAGE : CO-RE 96 Head Tip Pick Up (Single Step) - complete; > CO-RE 96 Head: HT_L_0001, > channel 1: 1 > channel 2: 2 > channel 3: 3 > channel 4: 4 > channel 5: 5 > channel 6: 6 > channel 7: 7 > channel 8: 8 > channel 9: 9 > channel 10: 10 > channel 11: 11 > channel 12: 12 > channel 13: 13 > channel 14: 14 > channel 15: 15 > channel 16: 16 > channel 17: 17 > channel 18: 18 > channel 19: 19 > channel 20: 20 > channel 21: 21 > channel 22: 22 > channel 23: 23 > channel 24: 24 > channel 25: 25 > channel 26: 26 > channel 27: 27 > channel 28: 28 > channel 29: 29 > channel 30: 30 > channel 31: 31 > channel 32: 32 > channel 33: 33 > channel 34: 34 > channel 35: 35 > channel 36: 36 > channel 37: 37 > channel 38: 38 > channel 39: 39 > channel 40: 40 > channel 41: 41 > channel 42: 42 > channel 43: 43 > channel 44: 44 > channel 45: 45 > channel 46: 46 > channel 47: 47 > channel 48: 48 > channel 49: 49 > channel 50: 50 > channel 51: 51 > channel 52: 52 > channel 53: 53 > channel 54: 54 > channel 55: 55 > channel 56: 56 > channel 57: 57 > channel 58: 58 > channel 59: 59 > channel 60: 60 > channel 61: 61 > channel 62: 62 > channel 63: 63 > channel 64: 64 > channel 65: 65 > channel 66: 66 > channel 67: 67 > channel 68: 68 > channel 69: 69 > channel 70: 70 > channel 71: 71 > channel 72: 72 > channel 73: 73 > channel 74: 74 > channel 75: 75 > channel 76: 76 > channel 77: 77 > channel 78: 78 > channel 79: 79 > channel 80: 80 > channel 81: 81 > channel 82: 82 > channel 83: 83 > channel 84: 84 > channel 85: 85 > channel 86: 86 > channel 87: 87 > channel 88: 88 > channel 89: 89 > channel 90: 90 > channel 91: 91 > channel 92: 92 > channel 93: 93 > channel 94: 94 > channel 95: 95 > channel 96: 96
2025-04-04 13:06:54.138 Microlab® STAR / VANTAGE : CO-RE 96 Head Aspirate (Single Step) - start;
2025-04-04 13:06:54.140 Microlab® STAR / VANTAGE : Main - error; An error occurred while running Vector. The error description is: The command requires 96 unused positions in the sequence R1_sequence_from_labware_barcode, and only 0 remain. (0x28 - 0x2 - 0x802)
2025-04-04 13:06:54.140 Microlab® STAR / VANTAGE : CO-RE 96 Head Aspirate (Single Step) - complete with error; An error occurred while running Vector. The error description is: The command requires 96 unused positions in the sequence R1_sequence_from_labware_barcode, and only 0 remain. (0x28 - 0x2 - 0x802)
2025-04-04 13:06:54.140 Microlab® STAR / VANTAGE : CO-RE 96 Head Aspirate - complete with error; An error occurred while running Vector. The error description is: The command requires 96 unused positions in the sequence R1_sequence_from_labware_barcode, and only 0 remain. (0x28 - 0x2 - 0x802)
2025-04-04 13:06:54.140 SYSTEM : Method has been aborted by the system - complete;

Thanks,
Mike

8

Hi @mueller68 ,

I was able to reproduce the issue. The issue is because the max value is not set within the way the library acquires the default sequence.

image
image569×196 3.51 KB

To get around it, you need to add a SeqSetMax value after the sequence was retrieved.

image
image700×208 16 KB

Update:

The link has been updated with this bug removed.

2 Likes
9

Hi Brandon,

Thanks, I confirmed that fixed the issue with it. Appreciate your help.

Mike

3 Likes