https://www.neb.com/en-us/products/e1050-exo-cip-rapid-pcr-cleanup-kit
Looks very interesting. 5 min total of two different temps and purportedly you have cleaned up PCR reaction ready for Sanger or NGS sequencing.
Sounds too good to be true.
I haven’t used it yet, but NEB is the type of company that if you reach out for a sample kit they will send you one, then you can see for yourself!
Imho marketing to resell good old exonuclease I. Won’t help you with primer dimers and has been used to prepare sanger reactions since the 80s. If your only concern is residual primers and dNTPS you can use it, but will get it much cheaper elsewhere.
1 Like