Hi all, I’m trying to automate cell straining processes on liquid handlers for downstream flow cytometry (with access to Opentrons Flex and Hamilton Star). Are there any automation friendly 96-well based cell strainers? What is an appropriate pipetting speed for cell straining? Thanks in advance!
This looks to be similar material/pore size to the strainer caps for tubes:
Multiscreen® 96 well Plate, mesh nylon membrane pore size 40 μm, non-sterile | Sigma-Aldrich
They mention letting the cell suspension strain via gravity flow or gentle centrifugation
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We use this with great success:
CSCRIE
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That looks amazing! Does it flow well by gravity or do you force the cell suspension through?
Pressure/Centrifuge is required to push the cells through the strainer.