Hey all,
If the cLLD from the labware definition is equal to 0, and the method uses “Use cLLD from Labware definition”, what sensitivity is used?
We are experiencing false/too sensitive/early detection of liquid. Probably due to low humidity (~10%).
Any suggestions?
Hi @yunghans,
Selecting either 0 or 5 in the Labware Definition for Capacitive LLD will set the detection level to “Low” in the method at runtime. My suggestion would be to set cLLD sensitivities in the method commands rather than relying on the Labware Definition settings to ensure better control over each pipetting step. Additionally, for what you’re experiencing specifically, I suggest trying the “Low” or “Medium” sensitivities to see if that improves results. If the issue persists, consider switching to pLLD instead if using dry tips for these transfers. In some instances, vapors coming off the surface of the liquid or droplets on the side of the wells/tubes will causes the cLLD sensor to trigger before reaching the liquid surface, particularly when using higher sensitivities.
Thank you,
Dan
Hi all, hope everyone is doing great.
I have been looking for an indication on how to interpreat the sensitivity of the cLLD. From your last answer @DanHartman_Hamilton , it seems that a higher sensitivity will make the conductivity sensor trigger faster and thus potentially too early or even mid-air in case of uncontrolled humid environments?
Setting the sensitivity on Low could potentially help in elinimating false/too early detections?
Below a screenshot from the Venus manual that seems to recommend to increase sensitivity in case of smaller volumes, is that the correct interpretation?
Many thanks in advance !
Hi @weiyolan,
All of that is the correct interpretation. It is more difficult to detect smaller volumes of liquid, which often coincide with smaller containers in a labware, so the table from the manual focuses solely on that aspect. The description in the manual also mentions the conductivity of the liquid, which plays a role in that less conductive liquids will require a higher sensitivity for detection. Lastly, vapors and droplets can trigger cLLD early, so there are considerations regarding both sensitivity and that possible need to switch to pLLD or a Fixed Height. In the end, testing will be required to determine the exact LLD parameters to use for a given transfer.
Thank you,
Dan
Hi Dan, thanks for the quick confirmation!!
