Dispensing a gradient/incrementing volume by column

ettenmoors · February 13, 2024, 11:20pm

Hi,
Hoping for some help writing a method to aliquot solution from a trough and dispense in a gradient across a 96-well plate
I want the dispense to go like this:
Column 1 contains 100ul of solution
Column 2 contains 105ul
Column 3 contains 110ul
Etc

I have a method that increases the volume by 5ul for every well (method and trace file below) but can’t figure out how to make the volume change per column (or after every 8 wells?). Would really appreciate any and all advice.

Method:

Trace:
2024-02-14 10:15:43> Microlab® STAR : Initialize (Single Step) - start;
2024-02-14 10:15:43> Microlab® STAR : Initialize (Single Step) - complete; > channel 1: Waste, > channel 2: Waste, > channel 3: Waste, > channel 4: Waste, > channel 5: Waste, > channel 6: Waste, > channel 7: Waste, > channel 8: Waste,
2024-02-14 10:15:43> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 10:15:43> Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete; > channel 1: HT_L_0001, 1 > channel 2: HT_L_0001, 2 > channel 3: HT_L_0001, 3 > channel 4: HT_L_0001, 4 > channel 5: HT_L_0001, 5 > channel 6: HT_L_0001, 6 > channel 7: HT_L_0001, 7 > channel 8: HT_L_0001, 8
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0001, 1, 100 uL > channel 2: rgt_cont_120ml_a00_0001, 2, 105 uL > channel 3: rgt_cont_120ml_a00_0001, 3, 110 uL > channel 4: rgt_cont_120ml_a00_0001, 4, 115 uL > channel 5: rgt_cont_120ml_a00_0001, 5, 120 uL > channel 6: rgt_cont_120ml_a00_0001, 6, 125 uL > channel 7: rgt_cont_120ml_a00_0001, 7, 130 uL > channel 8: rgt_cont_120ml_a00_0001, 8, 135 uL
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, A1, 100 uL > channel 2: Nun_96_Fl_Lb_0001, B1, 105 uL > channel 3: Nun_96_Fl_Lb_0001, C1, 110 uL > channel 4: Nun_96_Fl_Lb_0001, D1, 115 uL > channel 5: Nun_96_Fl_Lb_0001, E1, 120 uL > channel 6: Nun_96_Fl_Lb_0001, F1, 125 uL > channel 7: Nun_96_Fl_Lb_0001, G1, 130 uL > channel 8: Nun_96_Fl_Lb_0001, H1, 135 uL
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0001, 9, 140 uL > channel 2: rgt_cont_120ml_a00_0001, 10, 145 uL > channel 3: rgt_cont_120ml_a00_0001, 11, 150 uL > channel 4: rgt_cont_120ml_a00_0001, 12, 155 uL
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 10:15:44> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 10:15:45> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, A2, 140 uL > channel 2: Nun_96_Fl_Lb_0001, B2, 145 uL > channel 3: Nun_96_Fl_Lb_0001, C2, 150 uL > channel 4: Nun_96_Fl_Lb_0001, D2, 155 uL > channel 5: Nun_96_Fl_Lb_0001, E2, 0 uL > channel 6: Nun_96_Fl_Lb_0001, F2, 0 uL > channel 7: Nun_96_Fl_Lb_0001, G2, 0 uL > channel 8: Nun_96_Fl_Lb_0001, H2, 0 uL
2024-02-14 10:15:45> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 10:15:45> SYSTEM : Execute method - complete;
2024-02-14 10:15:45> SYSTEM : End method - start;
2024-02-14 10:15:45> Microlab® STAR : End method command - start;
2024-02-14 10:15:45> Microlab® STAR : Clean up instrument - progress; Move up 1000ul channels
2024-02-14 10:15:45> Microlab® STAR : Clean up instrument - progress; Move up CO-RE 96 Head
2024-02-14 10:15:45> Microlab® STAR : Clean up instrument - progress; Move down auto load Y drive
2024-02-14 10:15:45> Microlab® STAR : Clean up instrument - progress; Move up 1000ul channels
2024-02-14 10:15:45> Microlab® STAR : Clean up instrument - progress; Move up CO-RE 96 Head
2024-02-14 10:15:45> Microlab® STAR : Clean up instrument - progress; Move down auto load Y drive
2024-02-14 10:15:45> Microlab® STAR : Clean up instrument - progress; Spreading channels
2024-02-14 10:15:45> Microlab® STAR : Clean up instrument - progress; Switch off loading lights
2024-02-14 10:15:45> Microlab® STAR : End method command - complete;
2024-02-14 10:15:45> SYSTEM : End method - complete;

BrandonBare_Hamilton · February 14, 2024, 2:57am

Hi @ettenmoors ,

Welcome to the community!

This is one of those rare times that you would want to use the first Array option in the volume parameter for Aspirate and Dispense. See below:

Use single array value sets the value in the array at the specified index for all channels. So you just need to use the loop counter to specify which array value to pipette with.

2024-02-13 21:51:45.774 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-13 21:51:46.085 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;  > channel 1: stf_l_0001, 1 > channel 2: stf_l_0001, 2 > channel 3: stf_l_0001, 3 > channel 4: stf_l_0001, 4 > channel 5: stf_l_0001, 5 > channel 6: stf_l_0001, 6 > channel 7: stf_l_0001, 7 > channel 8: stf_l_0001, 8
2024-02-13 21:51:46.101 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-13 21:51:46.590 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: rgt_cont_60ml_BC_A00_0001, 1, 100 uL > channel 2: rgt_cont_60ml_BC_A00_0001, 2, 100 uL > channel 3: rgt_cont_60ml_BC_A00_0001, 3, 100 uL > channel 4: rgt_cont_60ml_BC_A00_0001, 4, 100 uL > channel 5: rgt_cont_60ml_BC_A00_0001, 5, 100 uL > channel 6: rgt_cont_60ml_BC_A00_0001, 6, 100 uL > channel 7: rgt_cont_60ml_BC_A00_0001, 7, 100 uL > channel 8: rgt_cont_60ml_BC_A00_0001, 8, 100 uL
2024-02-13 21:51:46.605 Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-13 21:51:46.924 Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete;  > channel 1: Cos_96_Rd_0001, A1, 100 uL > channel 2: Cos_96_Rd_0001, B1, 100 uL > channel 3: Cos_96_Rd_0001, C1, 100 uL > channel 4: Cos_96_Rd_0001, D1, 100 uL > channel 5: Cos_96_Rd_0001, E1, 100 uL > channel 6: Cos_96_Rd_0001, F1, 100 uL > channel 7: Cos_96_Rd_0001, G1, 100 uL > channel 8: Cos_96_Rd_0001, H1, 100 uL
2024-02-13 21:51:46.928 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - start;
2024-02-13 21:51:47.002 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste,  > channel 5: Waste,  > channel 6: Waste,  > channel 7: Waste,  > channel 8: Waste, 
2024-02-13 21:51:47.009 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-13 21:51:47.252 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;  > channel 1: stf_l_0001, 9 > channel 2: stf_l_0001, 10 > channel 3: stf_l_0001, 11 > channel 4: stf_l_0001, 12 > channel 5: stf_l_0001, 13 > channel 6: stf_l_0001, 14 > channel 7: stf_l_0001, 15 > channel 8: stf_l_0001, 16
2024-02-13 21:51:47.265 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-13 21:51:47.618 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: rgt_cont_60ml_BC_A00_0001, 1, 105 uL > channel 2: rgt_cont_60ml_BC_A00_0001, 2, 105 uL > channel 3: rgt_cont_60ml_BC_A00_0001, 3, 105 uL > channel 4: rgt_cont_60ml_BC_A00_0001, 4, 105 uL > channel 5: rgt_cont_60ml_BC_A00_0001, 5, 105 uL > channel 6: rgt_cont_60ml_BC_A00_0001, 6, 105 uL > channel 7: rgt_cont_60ml_BC_A00_0001, 7, 105 uL > channel 8: rgt_cont_60ml_BC_A00_0001, 8, 105 uL
2024-02-13 21:51:47.631 Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-13 21:51:47.895 Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete;  > channel 1: Cos_96_Rd_0001, A2, 105 uL > channel 2: Cos_96_Rd_0001, B2, 105 uL > channel 3: Cos_96_Rd_0001, C2, 105 uL > channel 4: Cos_96_Rd_0001, D2, 105 uL > channel 5: Cos_96_Rd_0001, E2, 105 uL > channel 6: Cos_96_Rd_0001, F2, 105 uL > channel 7: Cos_96_Rd_0001, G2, 105 uL > channel 8: Cos_96_Rd_0001, H2, 105 uL
2024-02-13 21:51:47.898 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - start;
2024-02-13 21:51:47.958 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste,  > channel 5: Waste,  > channel 6: Waste,  > channel 7: Waste,  > channel 8: Waste, 
2024-02-13 21:51:47.966 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-13 21:51:48.222 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;  > channel 1: stf_l_0001, 17 > channel 2: stf_l_0001, 18 > channel 3: stf_l_0001, 19 > channel 4: stf_l_0001, 20 > channel 5: stf_l_0001, 21 > channel 6: stf_l_0001, 22 > channel 7: stf_l_0001, 23 > channel 8: stf_l_0001, 24
2024-02-13 21:51:48.234 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-13 21:51:48.586 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: rgt_cont_60ml_BC_A00_0001, 1, 110 uL > channel 2: rgt_cont_60ml_BC_A00_0001, 2, 110 uL > channel 3: rgt_cont_60ml_BC_A00_0001, 3, 110 uL > channel 4: rgt_cont_60ml_BC_A00_0001, 4, 110 uL > channel 5: rgt_cont_60ml_BC_A00_0001, 5, 110 uL > channel 6: rgt_cont_60ml_BC_A00_0001, 6, 110 uL > channel 7: rgt_cont_60ml_BC_A00_0001, 7, 110 uL > channel 8: rgt_cont_60ml_BC_A00_0001, 8, 110 uL
2024-02-13 21:51:48.599 Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-13 21:51:48.875 Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete;  > channel 1: Cos_96_Rd_0001, A3, 110 uL > channel 2: Cos_96_Rd_0001, B3, 110 uL > channel 3: Cos_96_Rd_0001, C3, 110 uL > channel 4: Cos_96_Rd_0001, D3, 110 uL > channel 5: Cos_96_Rd_0001, E3, 110 uL > channel 6: Cos_96_Rd_0001, F3, 110 uL > channel 7: Cos_96_Rd_0001, G3, 110 uL > channel 8: Cos_96_Rd_0001, H3, 110 uL
2024-02-13 21:51:48.879 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - start;
2024-02-13 21:51:48.951 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste,  > channel 5: Waste,  > channel 6: Waste,  > channel 7: Waste,  > channel 8: Waste, 
2024-02-13 21:51:48.957 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-13 21:51:49.221 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;  > channel 1: stf_l_0001, 25 > channel 2: stf_l_0001, 26 > channel 3: stf_l_0001, 27 > channel 4: stf_l_0001, 28 > channel 5: stf_l_0001, 29 > channel 6: stf_l_0001, 30 > channel 7: stf_l_0001, 31 > channel 8: stf_l_0001, 32
2024-02-13 21:51:49.234 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-13 21:51:49.592 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: rgt_cont_60ml_BC_A00_0001, 1, 115 uL > channel 2: rgt_cont_60ml_BC_A00_0001, 2, 115 uL > channel 3: rgt_cont_60ml_BC_A00_0001, 3, 115 uL > channel 4: rgt_cont_60ml_BC_A00_0001, 4, 115 uL > channel 5: rgt_cont_60ml_BC_A00_0001, 5, 115 uL > channel 6: rgt_cont_60ml_BC_A00_0001, 6, 115 uL > channel 7: rgt_cont_60ml_BC_A00_0001, 7, 115 uL > channel 8: rgt_cont_60ml_BC_A00_0001, 8, 115 uL
2024-02-13 21:51:49.604 Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-13 21:51:49.868 Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete;  > channel 1: Cos_96_Rd_0001, A4, 115 uL > channel 2: Cos_96_Rd_0001, B4, 115 uL > channel 3: Cos_96_Rd_0001, C4, 115 uL > channel 4: Cos_96_Rd_0001, D4, 115 uL > channel 5: Cos_96_Rd_0001, E4, 115 uL > channel 6: Cos_96_Rd_0001, F4, 115 uL > channel 7: Cos_96_Rd_0001, G4, 115 uL > channel 8: Cos_96_Rd_0001, H4, 115 uL
2024-02-13 21:51:49.871 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - start;
2024-02-13 21:51:49.931 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste,  > channel 5: Waste,  > channel 6: Waste,  > channel 7: Waste,  > channel 8: Waste, 
2024-02-13 21:51:49.938 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-13 21:51:50.220 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;  > channel 1: stf_l_0001, 33 > channel 2: stf_l_0001, 34 > channel 3: stf_l_0001, 35 > channel 4: stf_l_0001, 36 > channel 5: stf_l_0001, 37 > channel 6: stf_l_0001, 38 > channel 7: stf_l_0001, 39 > channel 8: stf_l_0001, 40
2024-02-13 21:51:50.232 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-13 21:51:50.591 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: rgt_cont_60ml_BC_A00_0001, 1, 120 uL > channel 2: rgt_cont_60ml_BC_A00_0001, 2, 120 uL > channel 3: rgt_cont_60ml_BC_A00_0001, 3, 120 uL > channel 4: rgt_cont_60ml_BC_A00_0001, 4, 120 uL > channel 5: rgt_cont_60ml_BC_A00_0001, 5, 120 uL > channel 6: rgt_cont_60ml_BC_A00_0001, 6, 120 uL > channel 7: rgt_cont_60ml_BC_A00_0001, 7, 120 uL > channel 8: rgt_cont_60ml_BC_A00_0001, 8, 120 uL
2024-02-13 21:51:50.604 Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-13 21:51:50.873 Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete;  > channel 1: Cos_96_Rd_0001, A5, 120 uL > channel 2: Cos_96_Rd_0001, B5, 120 uL > channel 3: Cos_96_Rd_0001, C5, 120 uL > channel 4: Cos_96_Rd_0001, D5, 120 uL > channel 5: Cos_96_Rd_0001, E5, 120 uL > channel 6: Cos_96_Rd_0001, F5, 120 uL > channel 7: Cos_96_Rd_0001, G5, 120 uL > channel 8: Cos_96_Rd_0001, H5, 120 uL
2024-02-13 21:51:50.875 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - start;
2024-02-13 21:51:50.943 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste,  > channel 5: Waste,  > channel 6: Waste,  > channel 7: Waste,  > channel 8: Waste,

ettenmoors · February 14, 2024, 3:25am

Hi Brandon,

Thanks for your reply! I actually tried that same method shortly after making this post, but thought I should leave it up to make sure I was using the most efficient method.
I have a follow-up question: My Microlab STAR has 4 channels. How do I stop the volume from changing every 4 wells (e.g.: A1, B1, C1, D1 = 100ul, E1, F1, G1, H1 = 105ul)?

2024-02-14 14:17:17> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:17> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 1 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 1 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 1 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 1 uL
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, A2, 1 uL > channel 2: Nun_96_Fl_Lb_0001, B2, 1 uL > channel 3: Nun_96_Fl_Lb_0001, C2, 1 uL > channel 4: Nun_96_Fl_Lb_0001, D2, 1 uL
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 2 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 2 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 2 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 2 uL
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, E2, 2 uL > channel 2: Nun_96_Fl_Lb_0001, F2, 2 uL > channel 3: Nun_96_Fl_Lb_0001, G2, 2 uL > channel 4: Nun_96_Fl_Lb_0001, H2, 2 uL
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 3 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 3 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 3 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 3 uL
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:18> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, A3, 3 uL > channel 2: Nun_96_Fl_Lb_0001, B3, 3 uL > channel 3: Nun_96_Fl_Lb_0001, C3, 3 uL > channel 4: Nun_96_Fl_Lb_0001, D3, 3 uL
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 4 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 4 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 4 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 4 uL
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, E3, 4 uL > channel 2: Nun_96_Fl_Lb_0001, F3, 4 uL > channel 3: Nun_96_Fl_Lb_0001, G3, 4 uL > channel 4: Nun_96_Fl_Lb_0001, H3, 4 uL
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 5 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 5 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 5 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 5 uL
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, A4, 5 uL > channel 2: Nun_96_Fl_Lb_0001, B4, 5 uL > channel 3: Nun_96_Fl_Lb_0001, C4, 5 uL > channel 4: Nun_96_Fl_Lb_0001, D4, 5 uL
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 6 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 6 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 6 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 6 uL
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:19> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, E4, 6 uL > channel 2: Nun_96_Fl_Lb_0001, F4, 6 uL > channel 3: Nun_96_Fl_Lb_0001, G4, 6 uL > channel 4: Nun_96_Fl_Lb_0001, H4, 6 uL
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 7 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 7 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 7 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 7 uL
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, A5, 7 uL > channel 2: Nun_96_Fl_Lb_0001, B5, 7 uL > channel 3: Nun_96_Fl_Lb_0001, C5, 7 uL > channel 4: Nun_96_Fl_Lb_0001, D5, 7 uL
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 8 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 8 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 8 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 8 uL
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, E5, 8 uL > channel 2: Nun_96_Fl_Lb_0001, F5, 8 uL > channel 3: Nun_96_Fl_Lb_0001, G5, 8 uL > channel 4: Nun_96_Fl_Lb_0001, H5, 8 uL
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 9 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 9 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 9 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 9 uL
2024-02-14 14:17:20> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, A6, 9 uL > channel 2: Nun_96_Fl_Lb_0001, B6, 9 uL > channel 3: Nun_96_Fl_Lb_0001, C6, 9 uL > channel 4: Nun_96_Fl_Lb_0001, D6, 9 uL
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 10 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 10 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 10 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 10 uL
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, E6, 10 uL > channel 2: Nun_96_Fl_Lb_0001, F6, 10 uL > channel 3: Nun_96_Fl_Lb_0001, G6, 10 uL > channel 4: Nun_96_Fl_Lb_0001, H6, 10 uL
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 11 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 11 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 11 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 11 uL
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, A7, 11 uL > channel 2: Nun_96_Fl_Lb_0001, B7, 11 uL > channel 3: Nun_96_Fl_Lb_0001, C7, 11 uL > channel 4: Nun_96_Fl_Lb_0001, D7, 11 uL
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Dispense - complete;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate - start;
2024-02-14 14:17:21> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 14:17:22> Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete; > channel 1: rgt_cont_120ml_a00_0002, 1, 12 uL > channel 2: rgt_cont_120ml_a00_0002, 2, 12 uL > channel 3: rgt_cont_120ml_a00_0002, 3, 12 uL > channel 4: rgt_cont_120ml_a00_0002, 4, 12 uL
2024-02-14 14:17:22> Microlab® STAR : 1000ul Channel Aspirate - complete;
2024-02-14 14:17:22> Microlab® STAR : 1000ul Channel Dispense - start;
2024-02-14 14:17:22> Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 14:17:22> Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete; > channel 1: Nun_96_Fl_Lb_0001, E7, 12 uL > channel 2: Nun_96_Fl_Lb_0001, F7, 12 uL > channel 3: Nun_96_Fl_Lb_0001, G7, 12 uL > channel 4: Nun_96_Fl_Lb_0001, H7, 12 uL
2024-02-14 14:17:22> Microlab® STAR : 1000ul Channel Dispense - complete;

BrandonBare_Hamilton · February 14, 2024, 2:39pm

Hi @ettenmoors ,

Ah in that case since you need the channels to pipette the same volume twice, you need to embed a loop of 2 around the array set so instead of 12 values, you have 24 in your array. See below:

2024-02-14 09:34:36.193 Microlab® STAR : Initialize (Single Step) - start;
2024-02-14 09:34:36.339 Microlab® STAR : Initialize (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste, 
2024-02-14 09:34:36.351 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-14 09:34:36.588 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;  > channel 1: stf_l_0001, 1 > channel 2: stf_l_0001, 2 > channel 3: stf_l_0001, 3 > channel 4: stf_l_0001, 4
2024-02-14 09:34:36.603 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 09:34:36.891 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: rgt_cont_60ml_BC_A00_0001, 1, 100 uL > channel 2: rgt_cont_60ml_BC_A00_0001, 2, 100 uL > channel 3: rgt_cont_60ml_BC_A00_0001, 3, 100 uL > channel 4: rgt_cont_60ml_BC_A00_0001, 4, 100 uL
2024-02-14 09:34:36.908 Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 09:34:37.112 Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete;  > channel 1: Cos_96_Rd_0001, A1, 100 uL > channel 2: Cos_96_Rd_0001, B1, 100 uL > channel 3: Cos_96_Rd_0001, C1, 100 uL > channel 4: Cos_96_Rd_0001, D1, 100 uL
2024-02-14 09:34:37.116 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - start;
2024-02-14 09:34:37.178 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste, 
2024-02-14 09:34:37.185 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-14 09:34:37.353 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;  > channel 1: stf_l_0001, 5 > channel 2: stf_l_0001, 6 > channel 3: stf_l_0001, 7 > channel 4: stf_l_0001, 8
2024-02-14 09:34:37.365 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 09:34:37.561 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: rgt_cont_60ml_BC_A00_0001, 1, 100 uL > channel 2: rgt_cont_60ml_BC_A00_0001, 2, 100 uL > channel 3: rgt_cont_60ml_BC_A00_0001, 3, 100 uL > channel 4: rgt_cont_60ml_BC_A00_0001, 4, 100 uL
2024-02-14 09:34:37.574 Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 09:34:37.725 Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete;  > channel 1: Cos_96_Rd_0001, E1, 100 uL > channel 2: Cos_96_Rd_0001, F1, 100 uL > channel 3: Cos_96_Rd_0001, G1, 100 uL > channel 4: Cos_96_Rd_0001, H1, 100 uL
2024-02-14 09:34:37.728 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - start;
2024-02-14 09:34:37.779 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste, 
2024-02-14 09:34:37.788 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-14 09:34:37.948 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;  > channel 1: stf_l_0001, 9 > channel 2: stf_l_0001, 10 > channel 3: stf_l_0001, 11 > channel 4: stf_l_0001, 12
2024-02-14 09:34:37.963 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 09:34:38.175 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: rgt_cont_60ml_BC_A00_0001, 1, 105 uL > channel 2: rgt_cont_60ml_BC_A00_0001, 2, 105 uL > channel 3: rgt_cont_60ml_BC_A00_0001, 3, 105 uL > channel 4: rgt_cont_60ml_BC_A00_0001, 4, 105 uL
2024-02-14 09:34:38.187 Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 09:34:38.345 Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete;  > channel 1: Cos_96_Rd_0001, A2, 105 uL > channel 2: Cos_96_Rd_0001, B2, 105 uL > channel 3: Cos_96_Rd_0001, C2, 105 uL > channel 4: Cos_96_Rd_0001, D2, 105 uL
2024-02-14 09:34:38.348 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - start;
2024-02-14 09:34:38.412 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste, 
2024-02-14 09:34:38.420 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - start;
2024-02-14 09:34:38.588 Microlab® STAR : 1000ul Channel Tip Pick Up (Single Step) - complete;  > channel 1: stf_l_0001, 13 > channel 2: stf_l_0001, 14 > channel 3: stf_l_0001, 15 > channel 4: stf_l_0001, 16
2024-02-14 09:34:38.600 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
2024-02-14 09:34:38.801 Microlab® STAR : 1000ul Channel Aspirate (Single Step) - complete;  > channel 1: rgt_cont_60ml_BC_A00_0001, 1, 105 uL > channel 2: rgt_cont_60ml_BC_A00_0001, 2, 105 uL > channel 3: rgt_cont_60ml_BC_A00_0001, 3, 105 uL > channel 4: rgt_cont_60ml_BC_A00_0001, 4, 105 uL
2024-02-14 09:34:38.813 Microlab® STAR : 1000ul Channel Dispense (Single Step) - start;
2024-02-14 09:34:38.976 Microlab® STAR : 1000ul Channel Dispense (Single Step) - complete;  > channel 1: Cos_96_Rd_0001, E2, 105 uL > channel 2: Cos_96_Rd_0001, F2, 105 uL > channel 3: Cos_96_Rd_0001, G2, 105 uL > channel 4: Cos_96_Rd_0001, H2, 105 uL
2024-02-14 09:34:38.979 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - start;
2024-02-14 09:34:39.037 Microlab® STAR : 1000ul Channel Tip Eject (Single Step) - complete;  > channel 1: Waste,  > channel 2: Waste,  > channel 3: Waste,  > channel 4: Waste,