Hi there,
I am working on a program to process few plates from a stack. See below as the example, it successfully processed the first plate and put it to the final stack, however when it completed processing the second plate it gave me error “Step lost on Z-drive” when it was trying to stack the second plate above the first completed one.
My first time encountering this error, does anyone had similar experience?
Thanks!
2024-12-10 11:27:01.019 Venus software version: 6.0.1.3380
2024-12-10 11:27:01.051 SYSTEM : Analyze method - start; Method file C:\Program Files (x86)\Hamilton\Methods\P&G\Microbiology\Speedway Protocol\XFtestwritting.hsl
2024-12-10 11:27:02.288 Microlab� STAR / VANTAGE : Communication - progress; Connection to instrument is created.
2024-12-10 11:27:08.898 SYSTEM : Analyze method - complete;
2024-12-10 11:27:10.991 SYSTEM : Start method - start;
2024-12-10 11:27:10.991 SYSTEM : Start method - progress; User name: fang.x.4
2024-12-10 11:27:10.991 SYSTEM : Start method - progress; Phoenix software version: 6.0.1.50042
2024-12-10 11:27:10.991 SYSTEM : Start method - progress; Database version: Standard
2024-12-10 11:27:10.991 SYSTEM : Start method - progress; Sample tracking: On
2024-12-10 11:27:10.991 SYSTEM : Start method - progress; Vector Database: On
2024-12-10 11:27:10.991 SYSTEM : Start method - progress; Vector Database connection: Database 'HamiltonVectorDB' on Microsoft SQL Server 'LOCALHOST\HAMILTON' with user 'Hamilton'
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - start;
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Microlab� STAR / VANTAGE software version: 6.0.1.50042
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Serial number of Instrument: 100097
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of main module: "S.1.03.00.0100 2023-06-07"
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of arm controller: "S.1.04.04.0106 2023-06-05 (XRP AX)"
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of light controller: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of mag-pip arm controller: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of periphery box: "S.1.00.01.0009 2022-08-17 (XRP BX)"
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of 1000�l channels: S.1.01.10.02 2022-01-31 (XRP PX), S.1.01.10.02 2022-01-31 (XRP PX), S.1.01.10.02 2022-01-31 (XRP PX), S.1.01.10.02 2022-01-31 (XRP PX), S.1.01.10.02 2022-01-31 (XRP PX), S.1.01.10.02 2022-01-31 (XRP PX), S.1.01.10.02 2022-01-31 (XRP PX), S.1.01.10.02 2022-01-31 (XRP PX)
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of 5ml channels: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of MagPip channels: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of labware handling channels: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of auto load: "S.2.00.01.0029 2023-06-06 (IDLoader)"
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of iSWAP: S.1.02.01.02 2021-05-04 (XRP R0)
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of temperature-controlled carrier 1: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of temperature-controlled carrier 2: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of CO-RE 96 Head: S.1.00.06.01 2022-10-06 (XRP H0)
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of pump station 1: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of pump station 2: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of pump station 3: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of nano dispenser: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of nano dispenser pressure unit: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of CO-RE 384 Head: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of tube gripper: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of camera channel: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of gel card gripper: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of punch card gripper: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of puncher module: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of twister decapper station 1 module 1: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of twister decapper station 1 module 2: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of twister decapper station 2 module 1: not installed
2024-12-10 11:27:11.429 Microlab� STAR / VANTAGE : Start method command - progress; Firmware version of twister decapper station 2 module 2: not installed
2024-12-10 11:27:22.797 Microlab� STAR / VANTAGE : Start method command - complete;
2024-12-10 11:27:22.813 SYSTEM : Start method - complete;
2024-12-10 11:27:22.813 SYSTEM : Execute method - start; Method file C:\Program Files (x86)\Hamilton\Methods\P&G\Microbiology\Speedway Protocol\XFtestwritting.hsl
2024-12-10 11:27:22.891 LIBRARY: HamiltonVantageCAPCOM : Connect - start; i_stringIpAddress = '192.168.1.3', i_intPort = 2000
2024-12-10 11:27:23.016 LIBRARY: HamiltonVantageCAPCOM : Connect - progress; Object created
2024-12-10 11:27:24.641 USER : Trace - complete; Successfully connected device to 192.168.1.3:2000
2024-12-10 11:27:24.641 LIBRARY: HamiltonVantageCAPCOM : Connect - complete;
2024-12-10 11:27:24.656 LIBRARY: HamiltonVantageCAPCOM : TurnOnFans - start;
2024-12-10 11:27:24.656 USER : Trace - complete; Locking manual hood control
2024-12-10 11:27:24.672 USER : Trace - complete; Fan speed sent: 35
2024-12-10 11:27:35.082 USER : Trace - complete; Successfully turned on fans
2024-12-10 11:27:35.082 LIBRARY: HamiltonVantageCAPCOM : TurnOnFans - complete;
2024-12-10 11:27:35.082 LIBRARY: HamiltonVantageCAPCOM : SetFanSpeed - start; i_intSpeed = 30
2024-12-10 11:27:35.097 USER : Trace - complete; Fan speed sent: 30
2024-12-10 11:27:35.504 USER : Trace - complete; Successfully set fan speed to 30
2024-12-10 11:27:35.504 LIBRARY: HamiltonVantageCAPCOM : SetFanSpeed - complete;
2024-12-10 11:27:35.504 Microlab� STAR / VANTAGE : Initialize (Single Step) - start;
2024-12-10 11:27:35.550 Microlab� STAR / VANTAGE : Clean up instrument - progress; The instrument now tries to clean up its devices and superfluous material. Some of these items might be mentioned even if they are not configured or used.
2024-12-10 11:27:35.629 Microlab� STAR / VANTAGE : Initialize (Single Step) - complete;
2024-12-10 11:27:35.629 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - start;
2024-12-10 11:27:36.253 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - complete; > ##: 1AMLPer0
2024-12-10 11:27:36.253 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - start;
2024-12-10 11:27:36.863 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - complete; > ##: 0AMLLer0
2024-12-10 11:27:36.894 SYSTEM : User Input Dialog - start; <Assay choice>, Available button(s): <OK>, <Cancel>, Default button: <Cancel>, Input row 1 - Prompt: <Which assay to run? (1) day0 initial count dilution (2) day1/2/5/7 dilutions (3)Spectromax read plates.>, Default value: <1>, Type: <Integer>, Minimum: <1>, Maximum: <3>
2024-12-10 11:27:50.949 SYSTEM : User Input Dialog - complete; <Assay choice>, Closed with: <OK (1)>, Input row 1 - Prompt: <Which assay to run? (1) day0 initial count dilution (2) day1/2/5/7 dilutions (3)Spectromax read plates.>, Value: <2>
2024-12-10 11:27:50.980 SYSTEM : HSLTipCountingLib - progress; Warning! Renamed tip counter 'ML_STAR.seq_Tips_Channels' to 'ML_STARseq_Tips_Channels' (reason: invalid characters within given tip counter name)
2024-12-10 11:27:51.089 Microlab� STAR / VANTAGE : Set Status Light (Single Step) - start;
2024-12-10 11:27:51.105 Microlab� STAR / VANTAGE : Set Status Light (Single Step) - complete; > Status: Wait for user input > Event: Set
2024-12-10 11:27:51.120 SYSTEM : Edit Sequence Dialog - start; <Edit Tip Count>, Prompt: <Set the first and the last position of the tip sequence as the actual current and count position of the tip sequence respectively.>
2024-12-10 11:27:53.877 SYSTEM : Edit Sequence Dialog - complete; <Edit Tip Count>
2024-12-10 11:27:53.877 Microlab� STAR / VANTAGE : Set Status Light (Single Step) - start;
2024-12-10 11:27:53.908 Microlab� STAR / VANTAGE : Set Status Light (Single Step) - complete; > Status: Wait for user input > Event: Reset
2024-12-10 11:27:53.955 USER : Trace - complete; User Assay Choice = 2: day 1/2/5/7 test plates preparation================
2024-12-10 11:27:53.955 USER : Trace - complete; one product per row, 2 replicates of the serial dilutions [-1 to -6] is performed
2024-12-10 11:27:53.955 USER : Trace - complete; last line as negative control
2024-12-10 11:27:53.970 Microlab� STAR / VANTAGE : Lock/Unlock Front Cover (Single Step) - start;
2024-12-10 11:27:55.642 Microlab� STAR / VANTAGE : Lock/Unlock Front Cover (Single Step) - complete; > Front Cover: unlocked
2024-12-10 11:27:55.658 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - start;
2024-12-10 11:27:55.798 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - complete; > ##: 1AMLPer0
2024-12-10 11:27:55.814 SYSTEM : User Output Dialog - start; <setting up the deck>, Available button(s): <OK>, Default button: <OK>, Message: <Please prepare and double-check the following things on the deck: 1. TSB reservior with more than 250ml volume @rack 50 position 1 2. Sampleplates 96 DW @ rack 35 position 1->2->3, fill the column of left wells with TSB 3. working plates(96well) @rack 69 4. refill all the pipette tips racks 5. Input samples number = #samples + #TSB wells, make it as divisible by 8 Click OK only after you set up the deck >
2024-12-10 11:27:57.188 SYSTEM : User Output Dialog - complete; <setting up the deck>, Closed with: <OK (1)>
2024-12-10 11:27:57.204 SYSTEM : User Input Dialog - start; <Assay Settings>, Available button(s): <OK>, Default button: <OK>, Input row 1 - Prompt: <Enter how many samples you are testing today?>, Default value: <1>, Type: <Integer>, Minimum: <1>, Maximum: <239>
2024-12-10 11:28:02.786 SYSTEM : User Input Dialog - complete; <Assay Settings>, Closed with: <OK (1)>, Input row 1 - Prompt: <Enter how many samples you are testing today?>, Value: <24>
2024-12-10 11:28:02.802 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - start;
2024-12-10 11:28:05.020 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - complete; > ##: 1AMLPer0
2024-12-10 11:28:05.020 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - start;
2024-12-10 11:28:05.629 Microlab� STAR / VANTAGE : Firmware Command (Single Step) - complete; > ##: 0AMLLer0
2024-12-10 11:28:05.645 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - start;
2024-12-10 11:28:22.933 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - complete; > SpectroPlate_Stack1_0006:
2024-12-10 11:28:22.949 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - start;
2024-12-10 11:28:38.637 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - complete; > WorkingPlate_Transport:
2024-12-10 11:28:38.652 USER : Trace - complete; Lid Transport with CORE Paddle
2024-12-10 11:28:38.668 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - start;
2024-12-10 11:28:55.188 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - complete; > WorkingPlate_withLid:
2024-12-10 11:28:55.205 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - start;
2024-12-10 11:29:00.896 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - complete; > LID_PARK:
2024-12-10 11:29:00.913 USER : Trace - complete; Lid Transport with CORE Paddle
2024-12-10 11:29:00.916 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - start;
2024-12-10 11:29:05.042 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - complete; > LID_PARK:
2024-12-10 11:29:05.056 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - start;
2024-12-10 11:29:10.737 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - complete; > WorkingPlate_withLid:
2024-12-10 11:29:10.756 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - start;
2024-12-10 11:29:14.854 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - complete; > WorkingPlate_Transport:
2024-12-10 11:29:14.862 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - start;
2024-12-10 11:29:24.912 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - complete; > Spectromax_FinalStack1_0001:
2024-12-10 11:29:24.943 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - start;
2024-12-10 11:29:30.913 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - complete; > SpectroPlate_Stack1_0005:
2024-12-10 11:29:30.929 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - start;
2024-12-10 11:29:46.589 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - complete; > WorkingPlate_Transport:
2024-12-10 11:29:46.605 USER : Trace - complete; Lid Transport with CORE Paddle
2024-12-10 11:29:46.621 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - start;
2024-12-10 11:30:03.176 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - complete; > WorkingPlate_withLid:
2024-12-10 11:30:03.187 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - start;
2024-12-10 11:30:08.848 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - complete; > LID_PARK:
2024-12-10 11:30:08.865 USER : Trace - complete; Lid Transport with CORE Paddle
2024-12-10 11:30:08.866 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - start;
2024-12-10 11:30:12.995 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - complete; > LID_PARK:
2024-12-10 11:30:13.003 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - start;
2024-12-10 11:30:18.699 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - complete; > WorkingPlate_withLid:
2024-12-10 11:30:18.723 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - start;
2024-12-10 11:30:22.818 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Get Plate (Single Step) - complete; > WorkingPlate_Transport:
2024-12-10 11:30:22.828 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - start;
2024-12-10 11:30:28.468 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - progress; Error handling waiting for manual recovery!
2024-12-10 11:33:16.802 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - error; > CO-RE Grip: 1, E:P8:02/62:Repeat
2024-12-10 11:33:16.834 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - progress; Error manually recovered by user.
2024-12-10 11:33:25.344 Microlab� STAR / VANTAGE : - progress; User action: Message box 'Abort command' message 'Are you sure you want to abort the run?' was closed with button 'Yes'.
2024-12-10 11:33:25.344 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - error; > CO-RE Grip: 1, E:P5:02/62:Abort
2024-12-10 11:33:25.376 Microlab� STAR / VANTAGE : Main - error; An error occurred while running Vector. The error description is: Step aborted. The method should be restarted. ([P5: Z drive, Step lost on Z drive.] [P8: Z drive, Step lost on Z drive.]) (0x28 - 0x1 - 0x804)
2024-12-10 11:33:25.376 Microlab� STAR / VANTAGE : 1000ul Channel CO-RE Grip Place Plate (Single Step) - progress; Error manually recovered by user.
2024-12-10 11:33:25.376 Microlab� STAR / VANTAGE : **1000ul Channel CO-RE Grip Place Plate (Single Step) - complete with error; An error occurred while running Vector. The error description is: Step aborted. The method should be restarted. ([P5: Z drive, Step lost on Z drive.] [P8: Z drive, Step lost on Z drive.])** (0x28 - 0x1 - 0x804) > Spectromax_FinalStack1_0002:
2024-12-10 11:33:25.376 SYSTEM : Method has been aborted by the user - complete;
2024-12-10 11:33:25.376 SYSTEM : Abort method - start;
2024-12-10 11:33:25.391 Microlab� STAR / VANTAGE : Abort requested command - start;
2024-12-10 11:33:25.391 Microlab� STAR / VANTAGE : Abort requested command - complete;
2024-12-10 11:33:25.391 Microlab� STAR / VANTAGE : Start Method Abort Handler command - start;
2024-12-10 11:33:25.797 Microlab� STAR / VANTAGE : Clean up instrument - progress; Remove gripped labware from CO-RE Grip
2024-12-10 11:33:26.414 Microlab� STAR / VANTAGE : Lock/Unlock Front Cover (Single Step) - progress; Start error handling in walkaway mode (no dialog).
2024-12-10 11:33:26.414 Microlab� STAR / VANTAGE : Main - error; An error occurred while running Vector. The error description is: Step canceled. User-defined error handling will be used. (C0: Process busy.) (0x28 - 0x1 - 0x80a)
2024-12-10 11:33:26.414 Microlab� STAR / VANTAGE : Lock/Unlock Front Cover (Single Step) - progress; Error automatically recovered depending of custom error configuration (Walk-Away).
2024-12-10 11:33:26.461 Microlab� STAR / VANTAGE : User input requested - progress; Error handling waiting for manual recovery!
2024-12-10 11:33:27.976 Microlab� STAR / VANTAGE : Clean up instrument - progress; Button 'Open Gripper' pressed, object will be released now.
2024-12-10 11:33:30.788 Microlab� STAR / VANTAGE : User input requested - progress; Error manually recovered by user.
