I am from a lab that analyses metabolites. We’re in the process of preparing a library of metabolites by purchasing them in solid form. Our plan is to dissolve the solid metabolites in a solution, create aliquots, and then evaporate these aliquots to obtain solid samples.
For example, if we buy 10mg of a compound, we would dissolve all of it in 1ml, make ten 100ul aliquots, and then evaporate the solution to get ten tubes, each containing 1mg of solid. We initially attempted to use a speedvac for this process, but it takes a long time to evaporate the solution, leading to the compound being in solution for several hours at room temperature. Something we should probably want to avoid, given certain compounds are likely to be unstable in solution.
I expect there are some people here who specialise in high-throughput synthesis of chemical libraries. If so, how do you generally evaporate the liquid from compounds after synthesis
water and DMSO. A quick question: is this a freeze dryer without a centrifugal force? If so have you ever noticed solvents melting in the tubes and hence the solution lost. Would you recommend DMSO as opposed to water given its more inert?
No, It is a centrifuge with vacuum and heat. I would love to use something more volatile so it evaporates faster but due to the nature of our process we have to use DMSO and water mixtures.