Hi all,
I’m just wondering if someone has experience using the 20 mL reservoirs. Do they fit in the 60 mL reagent carriers? What is the dead volume?
Thanks!
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Yes, the 20mL reservoirs fit in the 60mL reagent carriers. The trough needs about 400μL to cover the wave shaped wells in the trough, but from testing with water, the dead volume can be as low as ~100μL.
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Thank you for the reply!
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I use the 20mL clear reservoirs in my lab. They’re great, and the lab loves them. Just make sure you get the correct carrier (53646-01)
Using AMPure beads I can consistently get down to ~250 µL DV - there’s some sort of super slick PTFE in use that is unreal for recovery.
I’d say my only gripe is that they are considered irregular containers so - as far as I’ve tested - they lack the snap-to-carrier capability in layout. Next steps are to get some opaque reservoirs and some lids for photosensitive reagents and test those out as well.
I see what you are referring to with the snap to carrier. It does snap to the 50mL reagent carrier labware definition for the EMEA territory, RGT_CAR_5R60_A00, but not the stateside version, 53646-01. There are some slight differences in the carriers. I have submitted a request to get this corrected and clarified.
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Overall i think they are great.
Only real issue we had (have tried the black ones) was that the plastic coating is very hydrophobic, great for reducing adhesion but it can “push” liquids so that it lumps together in one end of the through.
We found that after aspiration of a PCR MM the trough was often empty in one end but had liquid in the other end.
Despite this i think they work great and I just ordered the non-opaque version to use in development.
Since its kind of related, is the lids removable by channels/“tip pick up”? Does it require anything else?
Just jumping on this thread. We had a old and copious stock of Roche- MagNA Pure LC Medium Reagent Tub 20ml troughs (03 004 082 001) which I believe are not available anymore. So once we’ve used all these up, I need to find an alternative. Will these Hamilton 20ml reservoirs provide similar dead volume (I really hoping I can throw them in and re-teach positions without needing to re-validate everything volume-wise)!
Hi @ClaireB,
Yes the Roche 20mL troughs are no longer available and the product is discontinued. The Hamilton 20mL troughs should be identical to them including the zigzag pattern at the bottom of the trough. On Hamilton instruments the Roche troughs required an aluminum adapter to stand upright but these do not.
Matt
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