Can anyone advise on how to use the quadrant function to re-format from 1 x 384wp > 4 x 96-wp in a variable pipetting loop?

Thanks!

Can anyone advise on how to use the quadrant function to re-format from 1 x 384wp > 4 x 96-wp in a variable pipetting loop?

Thanks!

1 Like

@teksta Which arm are you using for pipetting ? FCA or an MCA384 or MCA96?

Why a variable pipetting loop instead of a normal loop? Is the number of samples in the wells of the source 384wp variable or always 384 samples to transfer?

With an MCA having 96 tips mounted it should be relative easy, one just needs to aspirate from the 384wp with a different column & row offset combination at

each Aspirate Step for aspirating a different quadrant. And pipetting is much quicker if you have the chance to use an MCA to pipette from 1x 384wp to 4x 96wp

With FCA you will need to work with the well offset parameter and of course the pipetting will take much longer compareed to a MCA.

I wasn’t aware of the quadrant function, but trying it out in 4 loops with a loop counter named cycle:

quadrant(“384 Well[001]”,cycle,8,12) for cycle=[1,2,3,4] leads to result [0,1,16,17]

hence the result could be useful to add this value as a quadrantOffset to a well offset parameter in a script line as an additional offset for the different quadrants in the 384 well plate

alternatives for the quadrant() function with the same results [0,1,16,17] for loop cycle [1,2,3,4] would be either

If cycle = 1 then quadrantOffset = 0

If cycle = 2 then quadrantOffset = 1

If cycle = 3 then quadrantOffset = 16

If cycle = 4 then quadrantOffset = 17

programmed either with script FC lines or with the if() function. Not very elegant but it does the job.

or set the quadrant offset with the following mathematical formula:

quadrantOffset = ( floor((cycle-1) /2)*16 ) + ( (cycle-1) MOD 2 )

for loop cycle [1,2,3,4] the result are the same [0,1,16,17] as with the quadrant function.

With FCA a script to transfer all 384 samples from a 384wp to 4x 96wp could look like the following

1 Like