Fluent MCA Stamping Column Selection

Tecan
1

I am trying to do a simple stamp of 96 samples from one plate to another. Previously I have just been using the MCA for reagent transfer.

When I pickup 96 tips and then select the plate and wells, FC 3.5 indicates 2 aspirations from column 1 and 0 from column 12. During execution there is no issue and the plate is successfully stamped. I shrugged this off as a software bug initially, but the sample tracking report reflect the double transfer in column 1.

If I indicate 11 columns of tips (1-11) there is no issue. I’ve also confirmed the teach point is good and the little red dot is in the middle well.

Is this a labware definition issue, or do I have a setting incorrect for the MCA?

Thanks

MCA Stamp Error MM Titer
MCA Stamp Error MM Titer1147×617 30.2 KB

MCA Stamp Error MM Titer 11 cols
MCA Stamp Error MM Titer 11 cols963×338 14.4 KB

2

What’s under the Advanced section of the top Aspirate command?

3

MCA Stamp Error MM Titer_adv
MCA Stamp Error MM Titer_adv1035×786 39.3 KB

4

Does it occur for any 96 well plate labware definition or only for this specific labware type?

Check the X & Y Teaching Positions for Well 1 vs. Well 96 (delta of shown x/y values divided by 11 well gaps in x direction and divided by 7 well gaps in y direction) nicely result with an exact 9mm spacing per well so it matches the 9mm spacings of the MCA channels

5

This was the solution thanks!

I was so focused on the y-spacing that I’m used to worrying about for the FCA (and has a handy graphic) that I neglected manually checking the x spacing.

1 Like