Fluent MCA384 aspirating from top of variable liquid volume

Tecan
1

Hi all! New to Tecan. I am trying to get my Fluent MCA384 to aspirate from the top of the liquid in 384 well plates that already have the liquid when added to the Fluent worktable. Does anyone have commands/ liquid class/microscript that works for this?

Here’s what I tried, an applications specialist took a quick look and said it should work but it didn’t:

  1. Made a liquid class and set to aspirate from Z known position
  2. Made a user prompt in the script to set a variable “BCellSupVol” to the volume the user knows is in the plate
  3. Used set variable command with SetAttribute to set the attribute “Volume” to BCellSupVol for each plate. I used this expression in a loop with loop variable=cycle: SetAttribute(“B Cell Culture[”+cycle+“]”,“Well[1].Volume”, BCellSupVol)

How do I get the miscroscript to calculate the height to aspirate from based on a user input of volume? It looks like its calculating based off of “availableVolume” which I can’t seem to set from the set variable command.

Thanks!

2

What version of Fluent Control are you on?

3

Hi, so sorry for the delayed reply. Thought I was going to get an email when someone replied! I changed my settings so now I will.

I’m using 3.4.10