Hello,
Wondering if anyone has any experience, or data with using the Fluoreye for quantitating DNA yet? E.g. Qubit reagents. I’m not expecting it to be able to match a plate reader for performance, but I’m very interested to hear thoughts on it! 
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Isn’t this product brand new? Not sure if there is enough in field units for someone to chime in on.
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Yes, the product is new and we’re showcasing it at SLAS. Hopefully, in the near future, we will host a webinar on Fluoreye. I will have to look into what beta testing was done by my colleagues in Europe. Stateside, @NickHealy_Hamilton got a chance to set it up and assess it on the VANTAGE platform in preparation for training the internal teams. He was quite impressed by the device and its readiness for product launch!
For now though, please check out our website which has a video and flyer:
https://www.hamiltoncompany.com/automated-liquid-handling/small-devices/fluoreye
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Alright, Can you put 5 on the same deck at the same time and measure 5 different plates concurrently, as long as they are on the same carrier?
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For the initial launch, it will support 1 channel/device at a time. But we are eager for more feedback and ideas to make further improvements and multiple devices at a time has certainly come up! Please let me know the application and throughput needs so we understand your use case more!
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Yes… we use the qubit reagent that you can buy of the shelf and use it in our own qc for volume verification for the Tecan Fluent.
I have more info if you need, but send DM if needed
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We have created a script called ‘AirFCA QC test’ on our Tecan Fluent that we perform monthly to make sure the AirFCA is pipetting within our specified specification for Accuracy and CV for each AirFCA pipetting channel.
A deviation of <5.0 % is seen as a PASS, between 5.0% and 10.0% is seen as a WARNING and >10.0% is seen as a FAIL. If we have a fail then at least we have something to show for when we contact the vendor (never happened to this point).
We use the Qubit Flex (for strips of 8) for measurement.
Reagents = Qubit™ Flex System Verification Assay Kit - Catalog number: Q33254
Pipette 200µL blank buffer as standard 1
Pipette 200µL of Red or Green dye as Standard 2
Pipette a ratio for volume verification (we use 6 strips so 6 repeats for each channel):
X µL of blank buffer
200 - X µL of Red or Green dye
Measure standards QC-strips on the Qubit Flex and select the correct assay
2 Standards GREEN = dsDNA BR assay
2 Standards RED = RNA BR assay
3 Standards = Protein assay (possible, but not done frequently in our lab)
Mix all Qubit strips properly and spin down
Make sure the orientation of all strips is the same as during pipetting
Measure samples and export data
Used the exported measurement data to calculate the volumes for each pipetting action
In the attached picture you can see that the template we made gives all the required information.
Additional tabs for the raw Qubit data, volume calculations, interpolation plots and Report.
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Hi Eric, in the FluorEYE SensorSetValues settings what should the Power LED 1/2 be, in order to produce similar dsDNA results with other mainstream fluorometers such as Qubit?
Instead of comparing to another quantification you’d want to use a standard. You could use the standard that come with Quibit kits even! Serially dilute the standard a few times then take a few replicate measurements across all dilutions to figure out the dynamic range and then you’ll see better correlation.
Measurements like spec, nanodrop, are always going to have a few factors that can affect the final readings including contamination, protein content, and even concentration - so a test like this is important to run before samples.
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HI @MSMDG
First off, I’d like to echo everything @evwolfson laid out, as the standard curve is the source of truth in this scenario (RFU<>sample concentration).
Regarding the ability to toggle the LED intensity, this is the main lever available to you in order to modulate the resolution of your measurement range. The brighter the LEDs, the higher the resultant RFU reading (to an extent). If you use the higher end of the brightness setting, you’ll get more RFU per concentration unit and ultimately a higher resolution assay.
That said, depending on the chemistry, samples, volume etc you want to make sure you aren’t using too high an LED intensity for the particular assay. In some cases, you’ll lose a linear relationship between intensity and resultant RFU as you can eventually get diminishing returns on fluorescent signal.
You’d want to tune your brightness so that for your chemistry and samples, you have enough brightness to get an acceptable resolution against your standard curve, but not so bright that you lose linearity. Its going to be unique to your case.
Thanks.
-Nick
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