I was wondering if there is a way to set a limit to the volume pooled into a well. I want to limit the liquid volume to be transferred up to a specific volume. Is there a simple way to achieve this? thanks.
This is programing, I would say. You will have to add up the volumes you have transfered already and evaluate before each transfer whether adding the current volume would push the well above the threshold. If yes, you can start pipetting to the next well. It’s an easy loop operation and the switch can comfortably be done via sequence incrementation.
If you want to get around the calculation, you can set the liquid seeking height slightly below the one that corresponds to the volume you want in the well and then program an user-defined errorhandling for the unexpected liquid found error. This will only be an approximation, however, unless you always transfer the same volume, in which case you can set the seeking height to one transfer volume below the maximum you wish to have, which should result.in a fairly precise cutoff.
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