How to switch between two tip racks?

Opentrons
1

I want to have both 200 uL and 1000 uL tip racks on deck, and have the p1000_single dynamically select the best tip to use based on the dispense volume (e.g., use 200 uL for dispenses <50 uL, 1000 uL for dispenses >=50 uL). What API/python commands should I use - is there a way to switch between the racks while maintaining the unified/automatic tip rack tracking? So that I wouldn’t have to manually keep track of which well of the tip_rack to pick from whenever i call pick_up_tip()!

Could one use configure_nozzle_layout on the p1000_single and use the tip_rack arg/parameter to change the active tip rack?

2

Tip tracking is automatic. You don’t have to track it yourself.
Also when switching between pipettes this is automatically correct

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3

Thank you for the input! I was thinking of using the p1000 but with either 1000 uL or 200 uL tips to improve accuracy, based on the dispense volume…

1 Like
4

Ah I see. I think you could also make the loop
if volume < 50:
tip_rack = tips_200
else:
tip_rack = tips_1000

and then pick_up_tip(tip_rack)

but never tried

5

That looks like it could work! Might have to try this…

6

A few ways you could do this.

pipette = protocol.load_instrument(flex_1channel_1000, mount=‘Right’)

def _pick_up(tips):

pipette.tip_racks = tips

pipette.pick_up_tip()

_pick_up(tips_200)

There’s no way to input a volume into the pipette.pick_up_tip() command, so making your own function would be easier. The above way would input the tips being used, so you would have to tell it which tips you want each time you pick up tips. Otherwise you can input volume into that argument if that’s easier for them.

pipette = protocol.load_instrument(flex_1channel_1000, mount=‘Right’)

def _pick_up(vol):

pipette.tip_racks = tips_200 if vol < 50 else tips_1000

pipette.pick_up_tip()

_pick_up(100) # 100 = volume that will be aspirated once a tip is picked up