I have been making a cherry pick method to allow for sample dilutions using a method I had used before. On loading the plate is present and detected in the plate load array;
I am uncertain how this would occur as I have used a very similar method before without issue. The only difference is that I split the IDs into 2 new arrays prior to building the sequence arrays. I can provide more details if needed, I just wanted to ask to see if anyone had anything obvious to check.
I suspect it is due to the labware id name that includes a hyphen. If you try and set this labware id to a labware on the deck layout, you get the following error message:
Thanks for getting back to me. I have used this format for years without issue until now and just within this one step as it was working with a slightly different configuration within the same method. Within the same method I have a barcode used “MH-00000000-CDNA” & “MH-00000000-NRM” and it was working without error. these formats are also that same as many of the Illumina reagents.
I plan on doing some testing today as my fear is that moving between arrays is changing the format and might have to set back to string. I am continuing testing and will update if I find an answer.
I understand that you’ve been using the barcode format for some time, but if you set that value as the Labware ID in the sequence in the new method, then I would expect an error at runtime when it’s used in an aspirate or dispense step.
thanks for that. This gave me the idea of where the problem was, and it was a mix up in the arrays. if you look below it was supposed to use the barcode to pull the labware position ID, but was using wrong array. Top sequence has it correct, the bottom does not.
