Hi All,
Has anyone noticed differences in liquid class performance between 96-well plates and 0.5 mL tubes?
I optimized the liquid class using 0.5 mL tubes (for weighing purposes), and everything looked fine during testing. However, when I switched to using a Bio-Rad 96-well plate with the same surface empty liquid class, I observed droplets forming on the well walls—something that didn’t happen at all in the tubes. Has anyone else experienced this, or found a workaround?
- Are the bottoms of the tubes and well similar enough or could there be splashing happening on the bottom of the plate that wouldn’t happen with the tube
- Could there be some static in the plate that shoots droplets onto the side of the wells that wouldn’t happen as much in the tube (XY of dispensing relative to center, diameter of well, material has propensity to generate static)
The wells of the Bio-Rad plate and the 0.5 ml Sarstedt tube appear to be quite similar. That could be the case—I’ll look into it further. Thank you so much for your response!