Minimum amount of space needed for 2 channels to fit in a well?

We are looking at using these agilent DWP in portrait to try to fit two 1 mL tips in at a time to mix.

What is the minimum well dimensions for the Hamilton STAR that would be necessary?

Agilent square well DWPs have a well length of 8.23mm, less than half the length of the 48 well plate above. Length-wise, spacing is exactly double that of the 96 DWP at 18mm.

So in theory so long as you can pipette from two separate wells of the 96 square DWP then you should have clearance.

https://www.agilent.com/cs/library/brochures/br-microplate-dimension-guide-5994-5247en-agilent.pdf?srsltid=AfmBOoog3b-NZPiDGUlVatGQ51Bkpf2wcnbwheXVoqA7Ln9gGmMSijGb

Hi Joebot,

Thanks for the reply! I’m specifically looking to have ~ 3.5 mL of volume in each well, so that’s why we were looking at the rectangle well 5 mL DWP specifically – also it looks like the rectangle well could possibly fit two 1 mL channels in each well so we can properly mix the 3.5 mL.

Considered the 10 mL 24 well DWP, but the 48 wells works better for the throughput we are looking at.

The minimum distance of 1 mL channels on a STAR is 9 mm. If you’ve got that, plus a little extra so the tip fits in (less extra space for smaller tip sizes), you can have 2 channels in one well. As a rule of thumb: If you have half the well (eg 48) but still a standard SBS format, then 2 channels is likely possible.

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