Hi All,
When using a Bravo w/out a Benchel, how would one ask the gripper to move a lid from a reservoir, briefly store it on another DW labware, then put it back to the reservoir? ‘Delid’ does not work unless a platepad has been designated as an accessory/lid hotel, but I would like to use the DW plate that the lid would be stored on. I have each LW defined as ‘can be lidded’, mounted and dismounted, and also a lid labware defined.
Mmh, you cannot use delid without a lid hotel, but you can fake a lid with a shallow plate on a DW plate using stacks. Check out the video at this link.
The trick is creating a plate whose geometry is such as to make the shallow plate behave as lid when downstacked to another position:
I can share a test protocol if you are interested.
Another possibiity is to use the mount/unmount tasks, but those ones works well when you first need to mount a plate on another. It is typically used for placing filter plates on DW plates.
Thank you, Mauro, you’ve gone above and beyond with this explanation. I came up with a similar work-around, and the same conclusions regarding delidding and mount/unmounting. Sharing your script and LW definitions would be great! For those unfamiliar with the down- and upstacking on a Bravo, remember to bring the scan stack function(s) into the startup protocol:)
Hi @mauro /all,
I am having a similar issue as stated in this thread a couple years ago. I wanted to try to ask in this forum in case i could hopefully get some feedback from more experienced Bravo users
I have defined a profile where i have a lid hotel in 1 slot (the only slot available after all the plates and tip boxes i need on the deck). I have 2 plates that are delidded and relided throughout the protocol (that all works fine), but then I have another plate (2 ml deep well plate) that I need to cover just at the end (it can use any of the other 2 lids; the other plates can remain uncovered at the end, does not matter), but it does not let me relid this 1 plate if it does not have a lid on it first (and i dont have space to add another lid hotel). I was trying to handle this issue with the Mount command, using a lid as a labware directly, but I am not very familiar with this command (or with the Downstacking) and could not figure it out with the online documentation. What would be the easiest or most straightforward way of doing this? Could you maybe share some example script? Thanks a lot for the feedback in advance!
Best,
Aina
Hi! I think that using the lid as labware and the mount task is the most promising way. I’ll try to find some time to craft a test protocol tomorrow or Saturday.
In the meantime could you share the vzp of the protocol so that I can have an idea of how complex it is?
Hi Mauro! Thanks for you response! It does not let me upload vzp files nor a compressed zip file (authorized extensions: jpg, jpeg, png, gif, heic, heif, webp, avif, svg). How should I send you?
Best,
Aina
