Hi All,
When using a Bravo w/out a Benchel, how would one ask the gripper to move a lid from a reservoir, briefly store it on another DW labware, then put it back to the reservoir? ‘Delid’ does not work unless a platepad has been designated as an accessory/lid hotel, but I would like to use the DW plate that the lid would be stored on. I have each LW defined as ‘can be lidded’, mounted and dismounted, and also a lid labware defined.
Mmh, you cannot use delid without a lid hotel, but you can fake a lid with a shallow plate on a DW plate using stacks. Check out the video at this link.
The trick is creating a plate whose geometry is such as to make the shallow plate behave as lid when downstacked to another position:
I can share a test protocol if you are interested.
Another possibiity is to use the mount/unmount tasks, but those ones works well when you first need to mount a plate on another. It is typically used for placing filter plates on DW plates.
Thank you, Mauro, you’ve gone above and beyond with this explanation. I came up with a similar work-around, and the same conclusions regarding delidding and mount/unmounting. Sharing your script and LW definitions would be great! For those unfamiliar with the down- and upstacking on a Bravo, remember to bring the scan stack function(s) into the startup protocol:)
Hi @mauro /all,
I am having a similar issue as stated in this thread a couple years ago. I wanted to try to ask in this forum in case i could hopefully get some feedback from more experienced Bravo users
I have defined a profile where i have a lid hotel in 1 slot (the only slot available after all the plates and tip boxes i need on the deck). I have 2 plates that are delidded and relided throughout the protocol (that all works fine), but then I have another plate (2 ml deep well plate) that I need to cover just at the end (it can use any of the other 2 lids; the other plates can remain uncovered at the end, does not matter), but it does not let me relid this 1 plate if it does not have a lid on it first (and i dont have space to add another lid hotel). I was trying to handle this issue with the Mount command, using a lid as a labware directly, but I am not very familiar with this command (or with the Downstacking) and could not figure it out with the online documentation. What would be the easiest or most straightforward way of doing this? Could you maybe share some example script? Thanks a lot for the feedback in advance!
Best,
Aina
Hi! I think that using the lid as labware and the mount task is the most promising way. I’ll try to find some time to craft a test protocol tomorrow or Saturday.
In the meantime could you share the vzp of the protocol so that I can have an idea of how complex it is?
Hi Mauro! Thanks for you response! It does not let me upload vzp files nor a compressed zip file (authorized extensions: jpg, jpeg, png, gif, heic, heif, webp, avif, svg). How should I send you?
Best,
Aina
I am having similar difficulties figuring this out. I’ve tried making the lid it’s own piece of labware, but I’m running into limitations as only one Upstack and Downstack task is permitted per process lane, while I need multiple Relid and Delid operations. The lid begins the process tied to Plate 1, but partway through becomes stacked onto Plate 2. The plates themselves also move while delidded. I looked into the Mount and Dismount tasks, but it doesn’t seem possible to begin a process with the labware already mounted.
Hi!
One of the (old) tricks is virtually lidding the plate using the phantom robot and a virtual stacker.
Basically you have a process for the lid and another for your plate. You mount the plate with the lid using the phantom stacker and then you have your starting lidded plate that you can dismount if you need to
In the example above the source on loc 3 is virtually lidded with a lid coming from the phantom stacker (that is equivalent to saying that you have a plate at loc 3 that starts lidded). The lidded plate is moved to loc 1 and delidded. The lid is first moved to loc 9 then to loc 8 and the plate is moved to loc 5 and loc 1 where it is eventually lidded again. The lidded plate is finally moved to loc 5.
this is the Gantt chart:
While this method is useful for delidding without a lid hotel I could not find any clever way for passing the lid directly from one plate to another. Of course one can do something like this:
but a place plate for the lid between the two “wait for” is needed to avoid an error:
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And this is all I know about mount/dismount with lids!
m



