Hey everyone, just looking for some advice/insight/whatever else. First of all, I know that multi-dispensing 2uL into a 384 well plate using the 96 MPH is NOT recommended, but doing this would greatly speed up our run time so I’m trying everything I can before going back to the 8-span. So the first part of this is adding 8uL of qPCR MM into each well, which I have working great. Then I add 2uL of DNA (in water) to each well with a total of 3 dispenses, and my results have been very spotty. I’m not worried about volume accuracy at this point, I just want each well to have some DNA in it, which is currently not happening. There doesn’t seem to be a pattern of which wells get DNA and which don’t. I’ve attached pictures of the liquid class I’m using, the aspiration step, my first dispense back into the DNA plate, and the actual dispense step I’m having trouble with (these settings have worked the best out of all of them). Things I’ve tried include adjusting the dispense height (0.5mm, 1mm, 1.5mm, 2mm, and 3mm), side touch, jet dispense, and spinning down the MM before adding the DNA. Thanks in advance!
Try pre-wet tip. Also what is the humidity in your lab? as for lower volume humidity matters. Also play with swap speed
Sorry I should have mentioned, I have 3 cycles of tip pre-wetting on the aspiration step. Not sure about humidity, I’ll have to check that, thanks for the suggestion. I’ll try changing the swap speed and see if that helps, thanks!
what volume u r using for pre-wet?