im struggling to implement a multidispense with single steps.
I’ve tried a few things but when it comes to making sure each channel aspirates exactly what it dispenses over multiple wells i cant find a good solution, especially when sorting sequences for channel access (4 channel instrument).
How do you do the calculation for the Aspiration volumes and handle e.g. 3 Active Channels in the last transfer step.
Yeah just would be glad if one of you hab any suggestions or starting points.
Do you need to dispense different volumes for each position in the multidispense or for each channel?
I typically have my Dispense volume as a variable (or user input), then multiply that by the number of multidispense positions per channel to define the aspiration volume. You can use a single Aspirate step with your calculated Aspiration Volume, then loop over your dispense positions with single Dispense steps using your dispense volume. For the last step, Can you check how many positions are left in your controlling position and then if below 4, use channel patterns to only aspirate using the channels for the last remaining positions?
