New i-5 user couple questions

Beckman-Coulter
1

Hello! Does anyone know have a link to the updated Biomek software manual? I am having trouble finding it.

I created a program that has two Loops, one nested in the other. The first loop only aspirates a volume and the second loop dispenses the volume in a loop to different locations and stops when the first volume runs out. The first loop is ran again to aspirate more volume and return to dispensing.

The problem is the variable seems to reset when the second loop is exited. Since the variable is used to determine the plate location the program isn’t working correctly.

Is there a way to prevent or workaround the
loop variable getting
reset when the loop is exited?

Thanks!

2

@mgilson_beckman would you be able to assist here?

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3

can attachment the method

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4

I finally figured it out, but thanks everyone for the responses! Basically I added the first loop value to the second loop value to determine plate location. So it looked like this:

Loop1
Start: 1
End: =Number_of_Aliquots
Increment: INT(Tip_volume/volume)
Aspirate: = INT(Tip_volume/volume)*volume

Loop2
Start:1
End: INT(Tip_volume/volume)
Increment:1

Dispense:
Amount:=volume
Location: =((Loop1 variable-1)+Loop2 variable

There is also an if statement after the first loop but it wasn’t what had me stumped.

Thanks again! If anyone has a link to the i-5 biomek manual that would be great!

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