Hi, Lately I have experienced that several samples have no or too few reads after sequencing. I suspect its the pooling method that is the bad guy since we did a manual pooling afterwards and we got good results ! I have heard that the logfile HxUsbxComm tells us how far down the cannel goes down in the well befor it start to aspirate. Have someone her experience to interpret that kind of a file? The volume in the plate well is 15uL (3 mm heigth in the well) so the channels traveldistance has to very equal in each well I quess. It would be great if someone could interpret such a file could give me an explanation of Which of the numbers represents the distance the channels have traveled in each well?
Br Ann Kristin
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The HXCom is just a file that displays the firmware commands being send to the system and vice versa.
I wouldn’t spend time here unless you see the system doing other things. It’s like checking your car’s engine pistons and the valve clearance, whilst you just need to pump up the tires because these are flat.
Check
- Teaching of the system (to low on the bottom or to high?)
- Check liquidclass
- Check TADM
Another thing you can check - especially when using fixed height - is if the tip is still inside the liquid. TADM should be able to tell you this. Another option is to check your lab. Do you have AC? Is the humidity low or high?
And the final one are the tips? Are you using Hamilton tips? Good! Are you using cheap tips (purchase department might change this sometimes)…revert back.
I would go over this list, rather than checking firmware dumps.
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Hi Pascal, thank you so much for the answer and the explanation-I understand that I can’t use this file due to this type of error.