Pipetting to a 24w plate with a 96MPH head

Hamilton (Venus)
1

Hi all,

I was wondering if anyone else pipettes with a 96Head to a 24w plate. The best way I can find to do this right now is to trick the software and move virtually a 96w plate to where my 24w plate actually is.

Reading the " CO-RE 96 Head Pattern mode limitations" all modes seem to require a 9x9mm raster? Is there a better hack to get around this?

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2

Hi, @cwehrhan,
The advanced training states the following about this topic:

Pipetting with a 96-Channel Head for a 24-Well Plate

  1. General Requirements for 96-Channel to 24-Well Pipetting

    • Overlapping Labware: Ensure overlapping labware to align 96 sequence positions to the 24-well plate.

    • Raster Alignment: Use an 18mm raster for the 24-well plate to ensure optimal tip alignment.

    • Test Alignment: Verify alignment by using the 96-channel head (96MPH) and the desired tip pattern for teaching positions.

    • Tracking Limitations: Sample tracking may not accurately detect 24-well plate positions, and volume calculations/following will be unreliable.

    • Interaction with “Fake” 96-Well Labware: The 96MPH steps should interact with a virtual or “fake” 96-well labware overlay on the 24-well plate to maintain sequence conditions.

  2. Tip-to-Well Ratios and Applications: 4:1 or 2:1 Tips per Well (Overlapping with Snapped Labware)
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    • Tip Configuration: Use overlapping labware with either four or two tips per well.

    • Volume Capacity: Enables dispensing up to 4ml per well.

    • Well Considerations:

    • Round Wells: Mind the dispense height; tips may fit, but the channel barrels might not fully clear the well.

    • Square/Flat Bottom Wells: Provides better access to the bottom of the well.

    • Alternating Tips (2:1): Use either row-wise or column-wise alternation for washing applications.

    • Applications: Ideal for washing plates, e.g., BVS or MPE2.

  3. Tip-to-Well Ratios and Applications: 1:1 Tip-to-Well Ratio (Adjusted with Aligned Labware)

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    • Labware Positioning: Adjust the 96-well plate location so that A1 of both the 24-well and 96-well plates align.

    • Reliable Aspirate/Dispense: Enables bottom aspirate or dispense positions, though volume following and speed calculations may be inaccurate.

    • Channel Pattern Requirement: Must use a continuous channel pattern; alternate tips through physical arrangement if needed.

3

Ah yeah this is pretty much what I’m doing except with some tool to line up the A1 of both plates automatically. Was wondering if there was a better way since as they noted:

“volume following and speed calculations may be inaccurate”

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