I’m trying to carefully aspirate liquid off of a cell pellet and am concerned the speed at which the tips enter the liquid may be causing a disturbance.
Swap Speed seems to do this for the removal of the tips from the liquid, but not for entering.
I’ve seen a solution to this issue utilizing firmware commands, but is there any native way to do this in Venus?
It would be very useful if Venus had a validated motion control library that changed the speed of channel and iSWAP movements through firmware commands like that
As far as I know this would be a firmware command which you would toggle on before pipetting and off after. This would def be useful to have if this is something anyone with Hamilton could help out with that could be a great addition for our applications as well!
In the meantime you could turn off the Speed Up cLLD flag in settings but this is way too global of a change to be a permanent solution. Another “hack”, albeit very very resource intensive is using the star pipetting tools in a loop with a set timer delay each time you go down in Z.
Funny enough the inverse problem is present if you don’t power cycle a vantage after daily maintenance the pipetting tips move super slow in the Z direction globally.
Hamilton has the firmware switch for the carrier in and out, so it would be great to have this as a switch in the firmware as well.
What you might try doing is building a custom aspirate sub-method using firmware commands that
assigns a slower LLD search speed to the LLD detection step
slower submerge speed
standard aspirate
finally the slow retract.
Seems like a bit of work but you only really have to do it one time.
There are ways to do all of this using firmware commands but some of the parameters passed to the system via the standard steps are not exposed to the users. Case in point.
100%. Or a “Step builder” that allows you modify “ALL” of the available parameters for any given step and save them for future use. Much like the liquid classes.
I might be misinterpreting your needs, but there is a native command to drop the Tip Tracking Speed, which affects tip tracking in both aspirate and dispense steps. It doesn’t control how fast it enters the liquid, but controls how fast it moves down during aspiration, and has a resolution of 1/100th of the default speed.