Residual in tips after each action

Hello. We are seeing residual volumes in tips with sticky liquids (master mix, beads). I’ve tried various settings (flow rate, blowout, integrated mixing, aspirate/dispense mixing), but still a small amount is lost after each action (0.5-1 uL loss). It can be worse with multiple mix cycles (2-3 uL loss). This does not occur manually. We originally accepted this as a system limitation, but with new sensitive workflow, this loss is impacting the quality of our data.

I don’t want to add too much blowout to introduce air since the volumes are low, forcing a fixed height aspirate.

Using a Vantage (VoV) with standard CORE channels. Eagerly looking for suggestions and solutions. Thanks for your ideas.

Hi there,
are you worried about the pipetting accuracy or wasting the residual?
If it is the former: I’d overaspirate and discard an excess volume. E.g. If you need to dispense 10 µL, aspirate 12.5, dispense 10 to the target and discard the remaining 2.5. Usually helps a lot with pipetting accuracy from my experience.
best
Dominik