Hi, have you managed to use the smart commands? I supported US colleagues to set up a script remotely using smart commands. They have Fluent we have EVOs. We seem to have found a bug in 3.3 re residence time. If you increase residence time it should slow the flow rate but on timing the dispense step it actually increased it. We switched to using flow rate and method worked fine. Tecan say this bug is updated in 3.4
Iāve also worked with robocolumns on the Tecan fluent for the last 6 months, just ask if theres something i could possibly help with 
Hello, all wanted to jump on the thread, Iām pretty new to the TECAN and we have Fluent 780, been working with RoboColumns mostly, wanted to see if anyone has any tips; one of the main issues, Iām experiencing is the leaking from the top of the column. Using new columns, fresh material, one Liquid Class, which Iām afraid is ruining the o-rings inside by inserting the tip too fast. Regardless of how much I try to adjust the Z-dispense, I see various degrees of leaking. Any help would be great! Thanks!
is the leaking during the dispense ?
or after the dispense & when tips retract ?
What speeds are you currently dispensing at? Are these 600uL Robocolumns or 200uL? Do you see leaking for all stages of the process? I.e. do you see it only when loading cell harvest? Or do you see it when washing/eluting/cleaning as well?
Mostly during the dispense, I see it back pressuring more after the protein of interest is loaded.
Speeds are really important. Do you have 1mL syringes? 5mL? I found that speeds really needed to be under 5uL/s, preferably 1-2uL/s for most steps. The CIP solution could usually be loaded a bit quicker (~5uL/s).
Are you using cell harvest as your initial load? We found that some harvest needed to be filtered first, while others were OK straight out of the bioreactor. I guess it really depends on cells and how happy they are.
Right now, we have 5 mL syringes, but looking into possibly switching to 1.25 mL since the operating speeds are under 1 uL/s.
Hi, I have an issue that I get an error when I try to use the Te-Chrom smart command that āFound more than one instance of Te-Chrom Shuttleā. Have you ever seen this? Many thanks!
Hi, Iāve now managed to get all the different components of the Te-Chrom module working, and Iām trying to optimise my script so that I can simply enter the number of samples in the touchtool and the script will work out how to apply this to the script for each step. I think I need to set variables, if commands, loop commands, etc. As well as making clear touchtool script lines. but im already stuck on how i can manage this within the te-chrom smart commands. Has anyone made a script like this before and has any advice for me?
Have you already tried to apply your variable in the field Number of Samples below the liquid class at the bottom right of the TeChrom script command?
Hey, thanks for your response! That box is not present in my smart commandā¦I only have until āWait time [min]ā. Can you send me the manual that you found this in? Does it look like this on your FluentControl?
it is likely you will need a FluentControl upgrade to > v3.7 to get the latest Te-Chrom smart command updates
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My screenshot came from the manual of the latest version FC 3.8 SP1 (or go to FluentControl Help in the Menu). Probably it is a setting that was added in a later version.
The āNumber of Samplesā field is shown in the screenshot of the manual since version FC 3.6:
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