Hi, have you managed to use the smart commands? I supported US colleagues to set up a script remotely using smart commands. They have Fluent we have EVOs. We seem to have found a bug in 3.3 re residence time. If you increase residence time it should slow the flow rate but on timing the dispense step it actually increased it. We switched to using flow rate and method worked fine. Tecan say this bug is updated in 3.4
I’ve also worked with robocolumns on the Tecan fluent for the last 6 months, just ask if theres something i could possibly help with 
Hello, all wanted to jump on the thread, I’m pretty new to the TECAN and we have Fluent 780, been working with RoboColumns mostly, wanted to see if anyone has any tips; one of the main issues, I’m experiencing is the leaking from the top of the column. Using new columns, fresh material, one Liquid Class, which I’m afraid is ruining the o-rings inside by inserting the tip too fast. Regardless of how much I try to adjust the Z-dispense, I see various degrees of leaking. Any help would be great! Thanks!
is the leaking during the dispense ?
or after the dispense & when tips retract ?
What speeds are you currently dispensing at? Are these 600uL Robocolumns or 200uL? Do you see leaking for all stages of the process? I.e. do you see it only when loading cell harvest? Or do you see it when washing/eluting/cleaning as well?
Mostly during the dispense, I see it back pressuring more after the protein of interest is loaded.
Speeds are really important. Do you have 1mL syringes? 5mL? I found that speeds really needed to be under 5uL/s, preferably 1-2uL/s for most steps. The CIP solution could usually be loaded a bit quicker (~5uL/s).
Are you using cell harvest as your initial load? We found that some harvest needed to be filtered first, while others were OK straight out of the bioreactor. I guess it really depends on cells and how happy they are.
Right now, we have 5 mL syringes, but looking into possibly switching to 1.25 mL since the operating speeds are under 1 uL/s.