Sample Tracking and reports trough " Create Data Set"

Beckman-Coulter
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Hi everyone!

I’m relatively new to the Biomek world and am currently developing a protocol in Biomek 5. We’re implementing a workflow with a user-defined transfer pattern followed by dispensing into replicates. Since the destination wells are determined dynamically during the run, we need a way to track each sample from its source position to its final destination on a 384-well plate.

I started exploring the View Dataset functionality, hoping to use it to record where each sample ends up. However, I’m struggling to figure out how to create or populate a destination column when the destination positions aren’t predefined in the dataset but are instead generated during execution.

Has anyone implemented something similar? Is it possible to achieve this using the dataset functionality alone, or is there a better approach for tracking dynamically generated destination wells? If you’ve tackled a similar workflow, I’d really appreciate hearing how you approached it.

Thanks in advance!

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The view dataset step only allow viewing of local and global datasets and their state, at the point in the method in which the step is inserted. It’s a passive step.

To achieve what you need, use the create dataset step to allocate your sample IDs from a file to the source plate. If this step is configured correctly you should then see ‘SampleID’ (or whatever you’ve named the column header in your file) in the view dataset step for that source plate.

The Biomek software then automatically tracks the dataset when pipetting, so post dispense, you should see the SampleID values in the destination plate when viewing that plate with the view dataset step.

The hardest part here will be allocating the file columns correctly when creating the dataset. If you have no barcodes (or they haven’t been scanned by the software yet), then leave that dropdown blank.

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