Seeking Advice on Consistency Testing for Temperature Control Modules

Hi everyone,

I’m part of a team developing modules for pipetting workstations, including temperature control systems. Currently, we test the consistency of our temperature control module across 10 points on a 96-well aluminum block using a data logger and 10 PT100 probes. However, we’ve observed slight variations between tests, even when environmental conditions are tightly controlled. We’re uncertain whether these differences stem from measurement methods or the product itself.

Our current setup:

PT100 probes placed at fixed positions in the aluminum block.

Tests conducted at multiple setpoints (e.g., 4°C, 25°C, 37°C).

Environmental temperature stabilized prior to testing.

Questions for the community:

Practical Methods: How do you test temperature uniformity in similar systems (e.g., thermal cyclers, incubators)? Are there best practices for probe placement, calibration, or data analysis?

Troubleshooting: What factors might cause test-to-test variability despite controlled conditions (e.g., probe contact, sensor drift, thermal inertia)?

Standards: Are there industry standards (e.g., ISO, ASTM) or protocols specific to thermal performance validation in lab equipment?

Any insights on improving measurement reliability or references to relevant guidelines would be greatly appreciated!

Thanks in advance!