Hi all,
I have a Tecan EVO 200 with EVOware v2.7.
I generate .gwl scripts from scratch rather than using the FreedomEVOWare GUI.
I want to “Mix” (what’s called “Mix” in the EVOWare GUI) in a .gwl script. I was thinking this could be done with a series of aspirate + dispense commands.
More specifically, for a toy example, I have a 96-well plate. I want to aspirate up 50uL from rows A-H from column 1 (so all 8 tips should be used in parallel). Next, I want to dispense each 50uL volume into the corresponding well to the right in the second column (which already has 50uL of another solution inside). Lastly, I want to do 3 cycles of aspirating and dispensing 80uL (i…e, aspirate 80uL, dispense 80uL, aspirate 80uL, dispense 80uL, aspirate 80uL, dispense 80uL) from each of the 8 wells in the now-mixed second column, using all 8 tips in parallel.
How can I do this in a .gwl script?
I was thinking something like the following (only first two tips are shown but tips 3-8 would simply follow)…
A;Plate96-1;;96 Well Plate;1;;50;liquid_class_custom;;1
D;Plate96-1;;96 Well Plate;9;;50;liquid_class_custom;;1
A;Plate96-1;;96 Well Plate;9;;80;liquid_class_custom;;1
D;Plate96-1;;96 Well Plate;9;;80;liquid_class_custom;;1
A;Plate96-1;;96 Well Plate;9;;80;liquid_class_custom;;1
D;Plate96-1;;96 Well Plate;9;;80;liquid_class_custom;;1
A;Plate96-1;;96 Well Plate;9;;80;liquid_class_custom;;1
D;Plate96-1;;96 Well Plate;9;;80;liquid_class_custom;;1
W;
A;Plate96-1;;96 Well Plate;2;;50;liquid_class_custom;;2
D;Plate96-1;;96 Well Plate;10;;50;liquid_class_custom;;2
A;Plate96-1;;96 Well Plate;10;;80;liquid_class_custom;;2
D;Plate96-1;;96 Well Plate;10;;80;liquid_class_custom;;2
A;Plate96-1;;96 Well Plate;10;;80;liquid_class_custom;;2
D;Plate96-1;;96 Well Plate;10;;80;liquid_class_custom;;2
A;Plate96-1;;96 Well Plate;10;;80;liquid_class_custom;;2
D;Plate96-1;;96 Well Plate;10;;80;liquid_class_custom;;2
Is this possible, or will this cause issues due to there not being washing (i.e., W) in between each aspirate? If problematic, how else can I implement mixing? If not possible with basic aspirate+dispense commands, how can I do this with Advanced Worklist Commands (and can these be simply incorporated into a .gwl text file)?
Thanks in advance!
Evan
