Tecan Worklist (.gwl) - liquid handling with specific LiHa tips

Tecan
1

Hi,

I am interested in writing a worklist script for a Tecan FreedomEVO robot.

Minimal reproducible example in words:
I have a two 96-well plates. From the top 96-well plate in column 1, I want to pipette up 10uL into LiHa tip #1, 20uL into LiHa tip #2, and 30uL into LiHa tip #3. Next, I want to dispense them into the bottom 96-well plate as follows: Wells A1 and A2 will get 5uL from LiHa tip #1, wells B1 and B2 will get 10uL from LiHa tip #2, and wells C1 and C2 will get 15uL from LiHa tip #3.

In terms of a worklist (.gwl) text file, I have the following:

C;Aspirate
A;TopPlate;;96 Well Microplate - UBottom;1;;10;;;1
A;TopPlate;;96 Well Microplate - UBottom;2;;20;;;2
A;TopPlate;;96 Well Microplate - UBottom;3;;30;;;4
C;Dispense column 1
D;BottomPlate;;96 Well Microplate - UBottom;1;;5;;;1
D;BottomPlate;;96 Well Microplate - UBottom;2;;10;;;2
D;BottomPlate;;96 Well Microplate - UBottom;3;;15;;;4
C;Dispense column 2
D;BottomPlate;;96 Well Microplate - UBottom;9;;5;;;1
D;BottomPlate;;96 Well Microplate - UBottom;10;;10;;;2
D;BottomPlate;;96 Well Microplate - UBottom;11;;15;;;4

However, when I try importing and running this script, the UI provides an error that prevents me from running - it states that any line using the second or third tip is a “not available tip”. I am surprised it says not available, as I double checked and all eight tips are enabled under the Tip Selection section of the Worklist command menu.

Any insight on liquid handling with specific tips would be appreciated! Is there a simpler way to parallelize this, i.e., i.e., tips #1, 2, and 3 will go up and down at the same time because the aspirate and dispense commands are written such that all tips are positioned at the same appropriate column?

Some ideal function for me would hypothetically look like this:
A;TopPlate;;96 Well Microplate - UBottom;[position 1, position 2, position 3];;[volume 1, volume 2, volume 3];;;[tip 1, tip 2, tip 3,]
D;BottomPlate;;96 Well Microplate - UBottom;[position 1, position 2, position 3];;[volume 1, volume 2, volume 3];;;[tip 1, tip 2, tip 3,]

2

If you change the order of events, this should work

A;TopPlate;;96 Well Microplate - UBottom;1;;10;;;1
D;BottomPlate;;96 Well Microplate - UBottom;1;;5;;;1
D;BottomPlate;;96 Well Microplate - UBottom;9;;5;;;1
W;
A;TopPlate;;96 Well Microplate - UBottom;2;;20;;;2
D;BottomPlate;;96 Well Microplate - UBottom;2;;10;;;2
D;BottomPlate;;96 Well Microplate - UBottom;10;;10;;;2
W;
A;TopPlate;;96 Well Microplate - UBottom;3;;30;;;4
D;BottomPlate;;96 Well Microplate - UBottom;3;;15;;;4
D;BottomPlate;;96 Well Microplate - UBottom;11;;15;;;4
W;

the worklist grouping must be per tip - EVOware handles the command between each W;

the execution would aspirate tips 1, 2 & 3 in parallel
and dispense the same in Column 1
then column 2

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