Tecan Z-Heights Linked Between Different Tip Types

Hello!

I’m working on adjusting z-heights for the same labware type but for two different tip types. For example, the original z-max height for a 50 uL tip is 962 and 583 for a 1 mL tip. However, when I change the z-max height for a 50 uL tip to 950, the z-max height for the 1 mL tip is automatically changed to 571 (diff. = 12)

Is there any way for me to prevent this from happening? Or a way for me to adjust the tip height so that the z-max for both is ideal? Right now an ideal z-max for a 50 uL tip does not correspond to an ideal z-max for a 1 mL tip and vice-versa.

I use the Freedom EVOware Version 2.7.

Any advice/suggestions is extremely appreciated. Thank you!

The Z-max height isn’t ideal because you want the tips to have physically different Z-max heights? Or not ideal because you do want them to have the same physical Z-max but they currently are not?

Either way I’d point to your labware definitions for the tips themselves. I don’t believe Evoware has the option of using different heights based on tip being used. But you should be able to define different tip heights that could essentially get you the same thing. The problem being that if you want Z-max to be different but then you want Z-start to be the same, you’re gonna have problems.

Are you using Tecan branded tips? Or 3rd party vendor tips?

I would like the z-max height to be different for both tips, and would like to know how to change one without affecting the z-max height of the other. Right now, the z-max for the 50 uL tip is far from the bottom of the labware, but if I were to bring the 50 uL tip closer (e.g. from 962 to 970), then the z-max of the 1 mL tip will automatically change to 591 from 583 and will end up hitting the same labware when used.

I use 3rd party vendor tips. I have been adjusting z-heights in the labware definitions, but for some reason changing the z-max of a 50 uL tip will change the z-max of a 1 mL tip, even though I have set the tip type prior to editing.

for this, i would advise on using different labware compartments,

e.g. define Compartment 1 using 50 uL tip, define Compartment 2 using 1 mL tip

in the pipetting commands, you can define which compartment to pipette to/from

It sounds like your labware definitions for your tips are not 100% correct, which is gonna cause you a lot of frustration. I would first focus on getting the Z-max height dialed in such that both tip sizes go the exact same spot for Z-max and then implement what Optimize has suggested for achieving exactly what you want.

I believe the default DiTi labware is locked down in Evoware, so probably best to create copies and name them appropriately so you know which vendor’s tips apply to which DiTi labware definition. From there you should be able to tweak the definitions to get the tips better defined. In my experience, most people’s frustrations with Evoware is really improperly defined labware or carriers. Understanding how those are properly defined will save you a lot of work in the future.