Dear community,
I am currently developing a method containing a liquid liquid partitioning step via tip mixing.
After successfully testing a pipetting step including 20 mixing cycles before aspiration 0 µl, I increased the number of mixing cycles to 80. After the 80 cycles are performed, however, I get a hardware error 2/11 for all channels:
Microlab® STAR : 1000ul Channel Aspirate (Single Step) - start;
Microlab® STAR : 1000ul Channel Aspirate (Single Step) - progress; Error handling waiting for manual recovery!
Microlab® STAR : - progress; User action: Message box ‘Abort command’ message ‘Are you sure you want to abort the run?’ was closed with button ‘Yes’.
1000ul Channel Aspirate (Single Step) - error; > channel 1: PartitioningPlateOnLoadingPosition, A1, E:P1:02/11:Abort > channel 2: PartitioningPlateOnLoadingPosition, B1, E:P2:02/11:Abort > channel 3: PartitioningPlateOnLoadingPosition, C1, E:P3:02/11:Abort > channel 4: PartitioningPlateOnLoadingPosition, D1, E:P4:02/11:Abort > channel 5: PartitioningPlateOnLoadingPosition, E1, E:P5:02/11:Abort > channel 6: PartitioningPlateOnLoadingPosition, F1, E:P6:02/11:Abort > channel 7: PartitioningPlateOnLoadingPosition, G1, E:P7:02/11:Abort > channel 8: PartitioningPlateOnLoadingPosition, H1, E:P8:02/11:Abort
I ensured that the correction curve of the used liquid class is linear (0 - 0; 1000 - 1000) to not have accumulating plunger effects.
Any suggestions what the root cause of the error is and how to solve it? I am happy to provide COM trace files upon request.
Kind regards,
Martin