Hi!
I think I’m very close to having this working but I keep getting an error. This entire carrier is 300uL tips, want to verify that they are 300uL at the beginning of the script. It is already loading/unloading at the correct time and the Autoloader is scanning but I keep getting this error. Where is my mistake? Thank you!
Hello,
I can assist with this. But would you be able to give me a bit more detail and perhaps a trace log? Is this on a Vantage or a STAR? And if it is on a STAR, is your STAR equipped with a 1D or 2D autoload?
Matt
Thank you! Hamilton Star, 2D Autoloader
I can send the trace information in the morning, the error in the trace file was very vague
Here is the trace file showing the error that occurs after the Autoloader attempts to scan the tip racks
Since you are using the new 2D autoloader have you tried adding the specific ROI and illumination labware properties to each of your carriers? You can find them in the Venus 6 programmer’s manual or from the guide in the following link: 2D AL properties_VENUS 6-2 Software Programmer's Manual_en.pdf :: Hamilton
Each carrier on your deck layout may end up with 5 additional labware properties, otherwise the defaults for these parameters are applied instead and they may be inaccurate.
My apologies, I just realized I typoed - we have a 1D Autoloader, and are using Venus 4.
Hi,
My apologies for the delay but the 1D autoload makes all the difference. With the MFX tip isolator modules the barcode on a tip rack is roughly an additional 10mm higher in the z than on other standard tip carriers. This means that when the 1D scanner rotates to read horizontally the laser will not be in line with the barcodes. So these barcodes cannot be read without a 2D Autoload or Cognex attachment. See the image below:
No worries! Thank you very much for the response. I have the same settings in place for tip carriers of the standard height in your photo (to the right/forefront) that are yielding the same error when I go to try to scan them. The settings I am trying to use for those are exactly as I included in the screenshots as well. What detail am I missing to get this working with the standard height tip carriers?
Hi @Ham ,
Can you include a picture of your tips loaded in the tip carrier? With the settings listed, there should not be an issue. Just want to confirm there isn’t something else that is affecting the barcode scan.
Hi, I know it has been almost a year since this was posted, but you might be able to help me.
Using the guide you provided, I am trying to set the ROI to be close to the bottom of the labware, but have had no luck on getting the barcode labels to read.
We are using MFX carriers with deepwell plates. Any help would be greatly appreciated.
Hi @serg,
Are you using a 1D or a 2D autoload? And are you using a VANTAGE or STAR? While deepwell plates on an MFX carrier sound like they would not be a problem, there are just so many vairables to consider. So along with the aforementioned questions, what kind of barcode are you using (an example picture would help me immensely!)?
Matt
Hi @MatthewSmith_Hamilton we have a 2D autoload on a STARlet.
All good, ended up being the cognex scanner being too high, causing the viewfinder to be too high to scan the desired height.
Hi @serg,
Here is a section from the Venus 6 programmer’s manual:
A Y center offset of 20 means it will push the ROI 2mm to the right from the horizontal center. From there, the Y width of 860 means your ROI will be 86mm wide, or that the left and right boundaries of the ROI will be plus and minus 43mm from that 2mm offset from the horizontal center. But your Z center offset isn’t an offset from the center of the scanning window like the Y offset is. It’s an offset from the origin of the system, 100mm below deck. So a Z center offset of 45 is still technically putting the ROI under the baseplate of the system. This value should be more on the order of 2000 or somewhere around there: Take a look at the example below:
Try these out and let me know how it goes.
Matt
