Tip selection based on volume - Venus Hamilton

[Darren]

Hello,

We recently started using a Hamilton Starlet in our lab.

I have managed to create fairly simple methods and volume arrays, etc. from an Excel file. However, I am currently only using 300ul tips, but I would also like to be able to use 50ul tips when the volumes are lower.

Do you have any idea how to code this so that if the requested volume is 30ul, it will automatically take a 50ul tip, and above that, a 300ul tip?

Thanks in advance!

[EricSindelar_Hamilton]

There are some threads and examples on the forum. There are multiple approaches one can take using VENUS. In my opinion, the most straightforward would be to separate transfer volumes into an array for 50uL tip transfers and an array of 300uL tip transfers. The examples posted in the thread below demonstrate one way of accomplishing this:

Method Writing Exercises

You may want to check out this zip file that contains some VENUS challenges on arrays and barcoding. It includes a word document that reads like a set of basic user requirements and with each exercise, it adds more feature requests so you can iteratively build a more complex method. See below for an excerpt of Exercise 1: [image] The zip file contains completed examples of each exercise so you can compare. Part of the point of the exercise is to also anticipate what may be needed to make a m…

For something more advanced, you can refer to this thread. This thread provides some examples of how you can pick up different tip types and transfer the volumes together:

Tip Handling for Widely Varying Volumes

Wondering if anyone has a good solution for this problem: I’m trying to create a universal, one-size-fits-all solution to cherry picking using incoming worklists for plate mapping and transfer volumes. The issue that keeps coming up is the huge range of transfer volume that we can expect (anywhere from 1 uL to ~800 uL, essentially the full range of a STAR channel pipette). The difficulty is programming the robot to pick up appropriately sized tips for each transfer; what I’ve done so far is: s…

[Darren]

@EricSindelar_Hamilton Thank you very much, it works great!

[Darren]

Hello, I have a new question. When I sort my volumes by range during dispensing, the volumes are not dispensed according to their original index, which means, for example, A01 → A06 instead of A01. I imagine that I need to use an array with the initial index to control the dispensing?
Thank you in advance for your answers.