Total Tip Length Error

PyLabRobot User Discussion
1

I’m trying to set up my Tecan deck on the visualizer, and I seem to be getting some error about the tip length. Here’s my code:

from pylabrobot.liquid_handling import LiquidHandler
from pylabrobot.liquid_handling.backends import ChatterBoxBackend
from pylabrobot.liquid_handling.backends.tecan.EVO import LiHa, RoMa, EVO
from pylabrobot.visualizer.visualizer import Visualizer
from pylabrobot.resources.tecan import EVO150Deck
import asyncio
from pylabrobot.resources import (
    wash,
    tip_racks,
    tip_creators,
    tip_carriers,
    tecan_decks,
    plate_carriers,
    plates
)
from pylabrobot.liquid_handling.standard import (
  Pickup,
  Drop,
  Aspiration,
  Dispense,
  Move,
  GripDirection
)

lh = LiquidHandler(backend=ChatterBoxBackend(), deck=EVO150Deck())
lh.deck.get_resource("wash_station").unassign()

dt1 = tip_carriers.DiTi_SBS_3_Pos_MCA96(name = "tip rack carrier 1")
lh.deck.assign_child_resource(resource = dt1, rails = 6)

dt = tip_carriers.DiTi_SBS_4_Pos_MCA96(name = "tip rack carrier")
dt[3] = dttwo = tip_racks.DiTi_200ul_Filter_LiHa(name = "tip rack")
lh.deck.assign_child_resource(resource = dt, rails = 12)

plate = plate_carriers.MP_4Pos_flat(name = "plate carrier1")
plate[3] = plates.DeepWell_96_Well(name = "plate", with_lid= False)
plate[1] = plates.CaCo2_Plate_24_Well(name = "24 plate", with_lid = False)
plate[0] = plates.CaCo2_Plate_24_Well(name = "24 plate2", with_lid = False)
lh.deck.assign_child_resource(resource = plate, rails = 18)

station = wash.Wash_Station(name = "wash station")
lh.deck.assign_child_resource(resource = station, rails = 26)

dt2 = tip_carriers.DiTi_3Pos___Waste(name = "plate carrier2")
lh.deck.assign_child_resource(resource = dt2, rails = 27)

plate3 = plate_carriers.MP_3Pos_PCR(name = "plate carrier3")
plate3[0] = plates.DeepWell_96_Well(name = "well3", with_lid = False)
lh.deck.assign_child_resource(resource = plate3, rails = 33)

async def visualiser():
    vis = Visualizer(resource=lh)
    await vis.setup()

    evo = EVO(diti_count= 8)
    EVO.num_channels = 8
    evo.liha = LiHa(backend = evo, module = EVO.LIHA)
    op = Pickup(
        resource= dttwo.get_items("A1:H1"),
        offset= 0,
        tip=["A1:H1"]
    )
    #await evo.liha.get_disposable_tip(tips= [op], z_start = 877.0, z_search = 5.0)

asyncio.run(visualiser())

and here’s the terminal:

PS C:\Users\Name\Downloads\plr test> & "C:/Program Files/Python311/python.exe" "c:/Users/Name/Downloads/plr test/visualisertest.py"
Resource wash_station was unassigned from the robot.
Resource testack was assigned to the robot.
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
WARNING: total_tip_length <= 0.
Please get in touch at https://forums.pylabrobot.org/c/pylabrobot/23
Resource tip rack carrier was assigned to the robot.
Resource plate carrier1 was assigned to the robot.
Resource wash station was assigned to the robot.
Resource plate carrier2 was assigned to the robot.
Resource plate carrier3 was assigned to the robot.
Websocket server started at http://127.0.0.1:2121
File server started at http://127.0.0.1:1337 . Open this URL in your browser.
2

what is the physical length of the tip you’re using if you measure it with a ruler? How deep is the part that mounts onto the channel?

(It looks like the current total_tip_length in DiTi_200ul_Filter_LiHa_tip is defined as -5.0, and I’m not sure why (Wilson developed this). We have to update this to make things work)

The definition in the labware library file from evoware (Carrier.cfg) is 15;DiTi 200ul Filter LiHa;0;12/8/8;122/132/1112/762/132;1087/877/865/807;33.2;1;1;0/10/10;100;8388608;9;210;-50;1;0;0;121;128;5;4; if any (combination) of those numbers look familiar.

3

I currently have no access to the lab but I found it’s measurements in the product data sheet (in mm):

image
image356×674 23.5 KB

4

I can’t really match the data I see in carrier.cfg to any of those numbers. When do you think you can get the actual measurements? Also, could you share a link to that datasheet? Perhaps we can just pull resource definitions from there instead of carrier.cfg. dms open

5

Okay just got back from the lab.
Length of tip: 5.85cm
Part that mounts onto the channel: 2cm (hopefully I measured the right part)

Also, link to the datasheet.

1 Like