Bumping this rather than a new thread as I think I may be running into something related.
I’m trying to do an aspirate, dispense, mix cycle. It happens to be from/to two quadrants of a 384 plate and at low volume. My code looks like this:
await lh.aspirate96(lh.get_resource('alpha_plate').get_quadrant(3), volume=2.0,
flow_rate=2.0,
transport_air_volume=0,
pre_wetting_volume=0,
offset=Coordinate(x=1.5, y=1.5, z=-2.5))
await lh.dispense96(lh.get_resource('alpha_plate').get_quadrant(4), volume=2.0,
flow_rate=2.0,
jet=True,
offset=Coordinate(x=1.5, y=1.5, z=-0.5))
await lh.aspirate96(lh.get_resource('alpha_plate').get_quadrant(4), volume=0.0,
flow_rate=2.0,
transport_air_volume=0,
pre_wetting_volume=0,
homogenization_volume = 10,
homogenization_cycles = 2,
speed_of_homogenization = 10,
offset=Coordinate(x=1.5, y=1.5, z=-2))
When it gets to the second aspirate96 (which is the mix cycle, note volume=0.0, see Mixing during dispense - #4 by ben) it gives the following error:
---------------------------------------------------------------------------
STARFirmwareError Traceback (most recent call last)
Cell In[13], line 1
----> 1 await lh.aspirate96(lh.get_resource('alpha_plate').get_quadrant(4), volume=0.0,
2 flow_rate=1.0,
3 transport_air_volume=0.0,
4 pre_wetting_volume=0.0,
5 homogenization_volume = 10,
6 homogenization_cycles = 2,
7 speed_of_homogenization = 10,
8 offset=Coordinate(x=1.5, y=1.5, z=-2))
File ~/Documents/FutureHouse/Lab/Automation/pylabrobot/pylabrobot/liquid_handling/liquid_handler.py:1456, in LiquidHandler.aspirate96(self, resource, volume, offset, flow_rate, blow_out_air_volume, **backend_kwargs)
1454 well.tracker.rollback()
1455 channel.get_tip().tracker.rollback()
-> 1456 self._trigger_callback(
1457 "aspirate96",
1458 liquid_handler=self,
1459 aspiration=aspiration,
1460 error=error,
1461 **backend_kwargs,
1462 )
1463 else:
1464 for channel, well in zip(self.head96.values(), wells):
...
1208 he.errors[module_name].message += \
1209 " (call lh.backend.request_name_of_last_faulty_parameter)"
-> 1211 raise he
STARFirmwareError: {'CoRe 96 Head': HardwareError('Maximum volume in tip reached')}, C0EAid0013er99/00 H002/53
Output is truncated. View as a scrollable element or open in a text editor. Adjust cell output settings...
It seems like for some reason it thinks there is still volume in the tip after the prior dispense.
Some other details:
If I run the mix block (aspirate with volume=0) by itself, it works fine.
I swear that I ran the whole block at least once and it worked fine and has now decided to not work. I have no idea what that means, and I could be wrong.