Working with Echo plates (LP-0200) using CO-RE 96

Hamilton (Venus)
1

Hello ALL,

Have you ever had any experience working with 001-12782 (LP-0200) using only CO-RE 96? What tips have you used, liquid classes, carriers anything…?

We did work only with 50μl Standard Tips, because they match the wells when the head goes down into it… This tips are shorter than 300μl Standard Tips and for our needs they’d be more helpful…so we tried 300μl Standard Tips but had no result, because when the tips touched with wells they didn’t match and there was a sound of creaking… I hope you got it :slight_smile:

I’m just wondering if you’re working with this kinda stuff like this…

Thanks !!!

2

u can try Slim 300 tips if that is helpful. Also it seems that most likely the plate defiantion is incorrect.

3

We have also Slim 300 tips, they’re more worse than Standard tips.We got plate definition from Hamilton support tho…

4

To me, this sounds like a wrong teach point of the labware on the deck. Did you recheck these?

5

I rechecked) and now I show you the photo

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Tips 50
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IMG_5159
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Tips 300
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IMG_5161
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Tips 300 slim

and I send you the file we got from supprot
Echo_384_COC (1).zip

If you’all want I can do the video too.
Tips 50 look pretty well, Tips 300 are creaking, Tips 300 slim are not recommended to use in 384 well plate at all (It says in Operator’s Manual page 42)

6

Oh, now I get where the creaking sound is coming from. It’s some of the larger tips scratching the inner wall of the wells. I initally thought it happened when the tips reached the bottom of the wells.
Hm, this is tricky, since the tips on the MPH are never aligned perfectly and I guess this is the reason why the slim tips are not recommended in combination with 384 well plates. Sorry for not being more helpful.

1 Like
7

Out of curiosity and if you can share this detail: why do you prefer switching to larger tips anyways?

8

From time to time we work with plates such as Matrix 1.4 or Micronic 1.4 and you can’t reach low volumes using Tips 50, but Tips 300 perfect match the bottom

9

Maybe you could use the “Side touch” function:

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You would need to spin down the plate afterwards, but maybe this is a starting point to a solution that works for you?

10

I just did this. The First step is that the tips reach “Touch-off at bottom height” which is for example 2 mm and the Second is the “Side touch”… I tried with 50tips and again it’s perfect… with others I’m scared)