We have an opentron 2 with an attached P20 multi-channel GEN2. I was wondering if it is possible to script up a protocol that would allow me to pick up 8 tips and go to a source plate and aspirate variable volumes (say 10uL from back-most channel, 5uL from the middle channels, and 10uL from the front channel)?
I was wondering if there was a hardware limitation as some existing documentation says that the P20 GEN2 multichannel has a single motor that may not allow for this to happen?
@positiveoption123 All of the channels on an 8-channel OT-2 pipette are controlled by one lead screw. If you look at the OT-2 pipette white paper, it has a diagram of an 8-channel pipette cut in half, which shows how the channels are all connected. So, there is a hardware limitation that would prevent you from aspirating/dispensing different volumes across channels.
Do you know if anyone has modified the OT-2 to allow for variable volumes? I’ve seen some crazy mods in the field so I wouldn’t be surprised if someone’s done it
I have never seen anyone do that type of modification. One of the system’s benefits is its ability to be modified, but I feel that for this type of implementation, you might as well build a new pipette from the ground up. I have seen people build various attachments for the gantry.
