Fluent Dynamic DiTi Handling

Tecan
1

I have a script that uses Dynamic DiTi Handling in Sample Transfer, Transfer Individual Volumes, and Load Worklist Commands.

I’m having some trouble with the worklist command selecting the smallest tip size (50uL filtered). It is defaulting to 200uL filter tips even at 5uL in the worklist and will size up to 1000uL if needed, but it will not select the 50uL tips.

I copy pasted the Dynamic DiTi Handling setting from my Sample Transfer commands where the 50uL tips are appropriately selected. I’ve cycled through all possible “DiTi Types” selections above the Dynamic Line.

Does anyone have some advice on troubleshooting this?

2

can you share a screenshot of the worklist command with DDH parameters??

3

worklist_DDH
worklist_DDH1042×421 22.9 KB

SampleTransfer_DDH
SampleTransfer_DDH685×413 13.6 KB

4

If you change the 50 uL tip limit to 30, does this over-come the issue ?
this matches the Help file

i am curious if the software is adding the 10 uL air gap volume which renders the 50 uL tip invalid for use (based on 40 uL limit)

5

That’s a good thought, I’ll give that a shot. I was thinking that airgap was accounted in the channel rather than the tip but worth trying.

6

Turns out you need a leading semicolon (and maybe space) for the first item in a DDH command in Load Worklists. I have just brute forced every setting combo I could come up with. It should read:

“; 40:FCA DiTi 50uL Filtered; 185:FCA DiTI 200uL Filtered;”

3 Likes
7

Interesting, is this in the manual?

I moved away from Dynamic DiTi handling because the behavior felt a bit unpredictable for some edge cases.

8

The manual does not show the quotes or the semicolon, I just got lucky throwing things at the wall.

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9

I knew about the quotations but I did not know about that leading semicolon.

I’ve never had to use that.

10

I’ve also never had to use a leading semicolon or quotations, unless something has changed in a recent software version I don’t believe you have to.

I have a topic about the stupid greek letter µ vs the mathematical symbol µ and how that causes issues with dynamic DiTi handling

The proper syntax should be “8:FCA DiTi 10µl;30:FCA DiTi 50µl Filtered;”
----- EDIT: without quotes, so 8:FCA DiTi 10µl;30:FCA DiTi 50µl Filtered;
Which means:
10µl tips will be used for anything up to 8 uL
50µl Filtered tips will be used for anything above 8 uL and up to 30 uL

I made a topic on this before, maybe it will help you. Granted, maybe something has changed in a more recent software version that invalidates my post :sweat_smile:

11

Thanks for linking your thread, I did not know about the Mu issue.

I’m running FC v3.5.7 and out of curiosity from your response I deleted the leading ; this morning and it failed to pick up the first tip selection when surrounded by quotations.

12

I have never needed quotations, have you tried it without that? Sorry, the quotes in my post were just that, quotes, not intended to be in the code. My bad

So

8:FCA DiTi 10µl;30:FCA DiTi 50µl Filtered;

13

If you hover the mouse over the parameter while there is a syntax error it displays an example (there without double-quotes)

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Other commands that have Dynamic DiTi Handling (Transfer Individual Volumes) contain an example in the text field by default (though there with double quotes), but one can copy it as an example syntax from there and adjust (& get the µ character this way)

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PS: [ALT]+230 returns the µ character too

14

I am very glad that they implemented this, it’s not like that in my old version of the software